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1l3a

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[[Image:1l3a.jpg|left|200px]]<br /><applet load="1l3a" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l3a, resolution 2.30&Aring;" />
 
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'''Structure of the plant transcriptional regulator PBF-2'''<br />
 
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==Overview==
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==Structure of the plant transcriptional regulator PBF-2==
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The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 A resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with beta-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C(4) symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression.
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<StructureSection load='1l3a' size='340' side='right'caption='[[1l3a]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1l3a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3A FirstGlance]. <br>
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1L3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3A OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3a OCA], [https://pdbe.org/1l3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3a RCSB], [https://www.ebi.ac.uk/pdbsum/1l3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3a ProSAT]</span></td></tr>
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==Reference==
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</table>
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A new family of plant transcription factors displays a novel ssDNA-binding surface., Desveaux D, Allard J, Brisson N, Sygusch J, Nat Struct Biol. 2002 Jul;9(7):512-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12080340 12080340]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/WHY1_SOLTU WHY1_SOLTU] Single-stranded DNA-binding protein that acts as a transcriptional activator of the pathogenesis-related gene PR-10a. Upon elicitation, binds a 30bp promoter sequence known as elicitor element response (ERE) and is required for PR-10a expression.<ref>PMID:10948264</ref> <ref>PMID:12080340</ref> <ref>PMID:14960277</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l3/1l3a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l3a ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Solanum tuberosum]]
[[Category: Solanum tuberosum]]
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[[Category: Allard, J.]]
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[[Category: Allard J]]
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[[Category: Brisson, N.]]
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[[Category: Brisson N]]
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[[Category: Desveaux, D.]]
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[[Category: Desveaux D]]
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[[Category: Sygusch, J.]]
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[[Category: Sygusch J]]
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[[Category: cyclic c4 symmetry]]
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[[Category: pbf-2]]
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[[Category: plant]]
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[[Category: single strand dna binding protein]]
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[[Category: transcriptionnal activator]]
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[[Category: whirly]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:53 2008''
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Current revision

Structure of the plant transcriptional regulator PBF-2

PDB ID 1l3a

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