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1lbh

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(New page: 200px<br /><applet load="1lbh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lbh, resolution 3.2&Aring;" /> '''INTACT LACTOSE OPERON...)
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[[Image:1lbh.gif|left|200px]]<br /><applet load="1lbh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lbh, resolution 3.2&Aring;" />
 
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'''INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG'''<br />
 
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==Overview==
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==INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG==
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The lac operon of Escherichia coli is the paradigm for gene regulation., Its key component is the lac repressor, a product of the lacI gene. The, three-dimensional structures of the intact lac repressor, the lac, repressor bound to the gratuitous inducer, isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed, with a 21-base pair symmetric operator DNA have been determined. These, three structures show the conformation of the molecule in both the induced, and repressed states and provide a framework for understanding a wealth of, biochemical and genetic information. The DNA sequence of the lac operon, has three lac repressor recognition sites in a stretch of 500 base pairs., The crystallographic structure of the complex with DNA suggests that the, tetrameric repressor functions synergistically with catabolite gene, activator protein (CAP) and participates in the quaternary formation of, repression loops in which one tetrameric repressor interacts, simultaneously with two sites on the genomic DNA.
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<StructureSection load='1lbh' size='340' side='right'caption='[[1lbh]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lbh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LBH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPT:ISOPROPYL-1-BETA-D-THIOGALACTOSIDE'>IPT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lbh OCA], [https://pdbe.org/1lbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lbh RCSB], [https://www.ebi.ac.uk/pdbsum/1lbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lbh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lb/1lbh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lbh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LBH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with IPT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LBH OCA].
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*[[Lac repressor|Lac repressor]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the lactose operon repressor and its complexes with DNA and inducer., Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P, Science. 1996 Mar 1;271(5253):1247-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8638105 8638105]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, G.]]
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[[Category: Chang G]]
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[[Category: Horton, N.C.]]
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[[Category: Horton NC]]
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[[Category: Kercher, M.A.]]
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[[Category: Kercher MA]]
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[[Category: Lewis, M.]]
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[[Category: Lewis M]]
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[[Category: Lu, P.]]
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[[Category: Lu P]]
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[[Category: Pace, H.C.]]
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[[Category: Pace HC]]
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[[Category: IPT]]
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[[Category: dna-binding]]
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[[Category: repressor]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:26:55 2007''
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Current revision

INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG

PDB ID 1lbh

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