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1lkf

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(New page: 200px<br /><applet load="1lkf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lkf, resolution 1.90&Aring;" /> '''LEUKOCIDIN F (HLGB) ...)
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[[Image:1lkf.gif|left|200px]]<br /><applet load="1lkf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lkf, resolution 1.90&Aring;" />
 
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'''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS'''<br />
 
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==Overview==
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==LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS==
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Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are, self-assembling, channel-forming proteins related in sequence and, function. In the alpha-hemolysin heptamer, the channel-forming, beta-strands and the amino latch make long excursions from the protomer, core. Here we report the crystal structure of the water soluble form of, LukF. In the LukF structure the channel-forming region folds into an, amphipathic, three-strand beta-sheet and the amino latch forms a, beta-strand extending a central beta-sheet. The LukF structure illustrates, how a channel-forming toxin masks protein-protein and protein-membrane, interfaces prior to cell binding and assembly, and together with the, alpha-hemolysin heptamer structure, they define the end points on the, pathway of toxin assembly.
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<StructureSection load='1lkf' size='340' side='right'caption='[[1lkf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LKF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lkf OCA], [https://pdbe.org/1lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lkf RCSB], [https://www.ebi.ac.uk/pdbsum/1lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lkf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/1lkf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lkf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LKF OCA].
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*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel., Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E, Nat Struct Biol. 1999 Feb;6(2):134-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048924 10048924]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Gouaux, J.E.]]
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[[Category: Gouaux JE]]
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[[Category: Kamio, Y.]]
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[[Category: Kamio Y]]
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[[Category: Nariya, H.]]
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[[Category: Nariya H]]
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[[Category: Olson, R.]]
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[[Category: Olson R]]
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[[Category: Yokota, K.]]
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[[Category: Yokota K]]
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[[Category: hemolysin]]
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[[Category: leukotoxin]]
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[[Category: pore-forming toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:37:37 2007''
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Current revision

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

PDB ID 1lkf

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