1lmb

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(New page: 200px<br /><applet load="1lmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lmb, resolution 1.800&Aring;" /> '''REFINED 1.8 ANGSTRO...)
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[[Image:1lmb.gif|left|200px]]<br /><applet load="1lmb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lmb, resolution 1.800&Aring;" />
 
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'''REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX'''<br />
 
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==Overview==
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==REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX==
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The crystal structure of the lambda repressor-operator complex has been, refined to an R-factor of 18.9% at 1.8 A resolution. This refinement, using data collected at low temperature, has revealed the structure of the, N-terminal arm and shows that the interactions of repressor with the two, halves of the pseudo-symmetric operator site are significantly different., The two halves of the complex are most similar near the outer edge of the, operator site (in a region where the lambda and 434 repressors make, similar contacts), but they become increasingly different toward the, center of the operator. There are striking differences near the center of, the site where it appears that the arm makes significant contacts to only, one half of the DNA site. This suggested a new way of aligning the, operator sites in phage lambda. The high resolution structure confirms, many of the previously noted features of the complex, but also reveals a, number of new protein-DNA contacts. It also gives a better view of the, extensive H-bonding networks that couple contacts made by different, residues and different regions of the protein, and reveals important new, details about the helix-turn-helix (HTH) region, and the positions of many, water molecules in the complex.
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<StructureSection load='1lmb' size='340' side='right'caption='[[1lmb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lmb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lrd 1lrd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMB FirstGlance]. <br>
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1LMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. This structure superseeds the now removed PDB entry 1LRD. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LMB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmb OCA], [https://pdbe.org/1lmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmb RCSB], [https://www.ebi.ac.uk/pdbsum/1lmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Refined 1.8 A crystal structure of the lambda repressor-operator complex., Beamer LJ, Pabo CO, J Mol Biol. 1992 Sep 5;227(1):177-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1387915 1387915]
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== Function ==
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[[Category: Enterobacteria phage lambda]]
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[https://www.uniprot.org/uniprot/RPC1_LAMBD RPC1_LAMBD] Repressor protein CI allows phage lambda to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein CI to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Beamer, L.J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Pabo, C.O.]]
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Check<jmol>
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[[Category: double helix]]
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<jmolCheckbox>
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[[Category: protein-dna complex]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lm/1lmb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:40:30 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lmb ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Escherichia virus Lambda]]
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[[Category: Large Structures]]
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[[Category: Beamer LJ]]
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[[Category: Pabo CO]]

Current revision

REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX

PDB ID 1lmb

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