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1lnq

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(New page: 200px<br /> <applet load="1lnq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lnq, resolution 3.30&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1lnq.gif|left|200px]]<br />
 
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<applet load="1lnq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lnq, resolution 3.30&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MTHK AT 3.3 A'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF MTHK AT 3.3 A==
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Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an, appropriate stimulus. Two general categories of ion channel gating are, defined by the initiating stimulus: ligand binding (neurotransmitter- or, second-messenger-gated channels) or membrane voltage (voltage-gated, channels). Here we present the structural basis of ligand gating in a K(+), channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal, structure of a K(+) channel (MthK) from Methanobacterium, thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains, (regulators of K(+) conductance) form a gating ring at the intracellular, membrane surface. The gating ring uses the free energy of Ca(2+) binding, in a simple manner to perform mechanical work to open the pore.
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<StructureSection load='1lnq' size='340' side='right'caption='[[1lnq]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lnq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. The February 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Potassium Channels'' by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_2 10.2210/rcsb_pdb/mom_2003_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LNQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnq OCA], [https://pdbe.org/1lnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lnq RCSB], [https://www.ebi.ac.uk/pdbsum/1lnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lnq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ln/1lnq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lnq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LNQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1LNQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LNQ OCA].
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure and mechanism of a calcium-gated potassium channel., Jiang Y, Lee A, Chen J, Cadene M, Chait BT, MacKinnon R, Nature. 2002 May 30;417(6888):515-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037559 12037559]
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[[Category: Large Structures]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Potassium Channels]]
[[Category: Potassium Channels]]
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[[Category: Single protein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Cadene, M.]]
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[[Category: Cadene M]]
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[[Category: Chait, B.T.]]
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[[Category: Chait BT]]
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[[Category: Chen, J.]]
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[[Category: Chen J]]
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[[Category: Jiang, Y.]]
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[[Category: Jiang Y]]
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[[Category: Lee, A.]]
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[[Category: Lee A]]
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[[Category: Mackinnon, R.]]
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[[Category: Mackinnon R]]
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[[Category: CA]]
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[[Category: helix bundle]]
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[[Category: membrane protein]]
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[[Category: rossman fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:03:20 2007''
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Current revision

CRYSTAL STRUCTURE OF MTHK AT 3.3 A

PDB ID 1lnq

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