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1lod

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[[Image:1lod.jpg|left|200px]]
 
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==INTERACTION OF A LEGUME LECTIN WITH TWO COMPONENTS OF THE BACTERIAL CELL WALL==
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The line below this paragraph, containing "STRUCTURE_1lod", creates the "Structure Box" on the page.
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<StructureSection load='1lod' size='340' side='right'caption='[[1lod]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lod]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LOD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MUR:MURAMIC+ACID'>MUR</scene></td></tr>
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{{STRUCTURE_1lod| PDB=1lod | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lod OCA], [https://pdbe.org/1lod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lod RCSB], [https://www.ebi.ac.uk/pdbsum/1lod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lod ProSAT]</span></td></tr>
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</table>
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'''INTERACTION OF A LEGUME LECTIN WITH TWO COMPONENTS OF THE BACTERIAL CELL WALL'''
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== Function ==
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[https://www.uniprot.org/uniprot/LECB_LATOC LECB_LATOC]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We describe herein the refined high resolution x-ray structures of two components of the bacterial cell wall, muramic acid and muramyl dipeptide complexed to isolectin I from Lathyrus ochrus seeds. In both complexes, only the ring hydroxyl oxygen atoms of the bound sugar establish direct hydrogen bonds with isolectin I, as in the case of all the previously determined monosaccharide-lectin complexes. In addition, the lactyl methyl of both components strongly interacts via hydrophobic contacts with the side chains of residues Tyr100 and Trp128 of isolectin I, which could explain the higher affinity of isolectin I for muramic acid as compared with glucose. These 2 residues, however, are not involved in the stabilization of the oligosaccharide-isolectin I complexes. The dipeptide (D-Ala-D-iGln) of the second component is in stacking interaction with the N-acetyl group of glucose and with loop Gly97-Gly98 of isolectin I. In addition to these van der Waals' contacts, the dipeptide interacts with the lectin via well ordered water molecules also. Superposition of the structures of the muramyl dipeptide complex and of the muramic acid complex shows that the glucose ring in the dipeptide compound is tilted by about 15 degrees in comparison with that of muramic acid. The fact that the lactyl group has the same confrontation in both components reveals that the lectin is stereospecific and recognizes only diastereoisomer S of this group, which better fits the saccharide-binding site.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lo/1lod_consurf.spt"</scriptWhenChecked>
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1LOD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOD OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Interaction of a legume lectin with two components of the bacterial cell wall. A crystallographic study., Bourne Y, Ayouba A, Rouge P, Cambillau C, J Biol Chem. 1994 Apr 1;269(13):9429-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8144527 8144527]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lod ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Lathyrus ochrus]]
[[Category: Lathyrus ochrus]]
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[[Category: Protein complex]]
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[[Category: Bourne Y]]
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[[Category: Bourne, Y.]]
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[[Category: Cambillau C]]
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[[Category: Cambillau, C.]]
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[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:07:00 2008''
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Current revision

INTERACTION OF A LEGUME LECTIN WITH TWO COMPONENTS OF THE BACTERIAL CELL WALL

PDB ID 1lod

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