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1lv0

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[[Image:1lv0.jpg|left|200px]]<br /><applet load="1lv0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lv0, resolution 2.00&Aring;" />
 
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'''Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide'''<br />
 
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==Overview==
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==Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide==
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Rab GTPases, key regulators of membrane targeting and fusion, require the covalent attachment of geranylgeranyl lipids to their C terminus for function. To elucidate the role of lipid in Rab recycling, we have determined the crystal structure of Rab guanine nucleotide dissociation inhibitor (alphaGDI) in complex with a geranylgeranyl (GG) ligand (H(2)N-Cys-(S-GG)-OMe). The lipid is bound beneath the Rab binding platform in a shallow hydrophobic groove. Mutation of the binding pocket in the brain-specific alphaGDI leads to mental retardation. Strikingly, lipid binding acts through a conserved allosteric switching mechanism to promote release of the GDI-Rab[GDP] complex from the membrane.
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<StructureSection load='1lv0' size='340' side='right'caption='[[1lv0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LV0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv0 OCA], [https://pdbe.org/1lv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lv0 RCSB], [https://www.ebi.ac.uk/pdbsum/1lv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lv0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GDIA_BOVIN GDIA_BOVIN] Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lv0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lv0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LV0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GER:'>GER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV0 OCA].
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*[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]]
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__TOC__
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==Reference==
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</StructureSection>
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Geranylgeranyl switching regulates GDI-Rab GTPase recycling., An Y, Shao Y, Alory C, Matteson J, Sakisaka T, Chen W, Gibbs RA, Wilson IA, Balch WE, Structure. 2003 Mar;11(3):347-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12623022 12623022]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alory, C.]]
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[[Category: Alory C]]
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[[Category: An, Y.]]
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[[Category: An Y]]
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[[Category: Balch, W E.]]
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[[Category: Balch WE]]
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[[Category: Chen, W.]]
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[[Category: Chen W]]
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[[Category: Gibbs, R A.]]
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[[Category: Gibbs RA]]
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[[Category: Matteson, J.]]
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[[Category: Matteson J]]
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[[Category: Sakisaka, T.]]
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[[Category: Sakisaka T]]
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[[Category: Shao, Y.]]
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[[Category: Shao Y]]
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[[Category: Wilson, I A.]]
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[[Category: Wilson IA]]
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[[Category: GER]]
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[[Category: SO4]]
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[[Category: protein-ligand complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:43 2008''
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Current revision

Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide

PDB ID 1lv0

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