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1lv0
From Proteopedia
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==Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide== | ==Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide== | ||
| - | <StructureSection load='1lv0' size='340' side='right' caption='[[1lv0]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='1lv0' size='340' side='right'caption='[[1lv0]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1lv0]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1lv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LV0 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv0 OCA], [https://pdbe.org/1lv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lv0 RCSB], [https://www.ebi.ac.uk/pdbsum/1lv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lv0 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/GDIA_BOVIN GDIA_BOVIN] Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lv0 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lv0 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Rab GTPases, key regulators of membrane targeting and fusion, require the covalent attachment of geranylgeranyl lipids to their C terminus for function. To elucidate the role of lipid in Rab recycling, we have determined the crystal structure of Rab guanine nucleotide dissociation inhibitor (alphaGDI) in complex with a geranylgeranyl (GG) ligand (H(2)N-Cys-(S-GG)-OMe). The lipid is bound beneath the Rab binding platform in a shallow hydrophobic groove. Mutation of the binding pocket in the brain-specific alphaGDI leads to mental retardation. Strikingly, lipid binding acts through a conserved allosteric switching mechanism to promote release of the GDI-Rab[GDP] complex from the membrane. | ||
| - | + | ==See Also== | |
| - | + | *[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]] | |
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| - | == | + | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bos taurus]] |
| - | [[Category: Alory | + | [[Category: Large Structures]] |
| - | [[Category: An | + | [[Category: Alory C]] |
| - | [[Category: Balch | + | [[Category: An Y]] |
| - | [[Category: Chen | + | [[Category: Balch WE]] |
| - | [[Category: Gibbs | + | [[Category: Chen W]] |
| - | [[Category: Matteson | + | [[Category: Gibbs RA]] |
| - | [[Category: Sakisaka | + | [[Category: Matteson J]] |
| - | [[Category: Shao | + | [[Category: Sakisaka T]] |
| - | [[Category: Wilson | + | [[Category: Shao Y]] |
| - | + | [[Category: Wilson IA]] | |
| - | + | ||
Current revision
Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide
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Categories: Bos taurus | Large Structures | Alory C | An Y | Balch WE | Chen W | Gibbs RA | Matteson J | Sakisaka T | Shao Y | Wilson IA
