1lvk

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(New page: 200px<br /><applet load="1lvk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lvk, resolution 1.9&Aring;" /> '''X-RAY CRYSTAL STRUCTU...)
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[[Image:1lvk.gif|left|200px]]<br /><applet load="1lvk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lvk, resolution 1.9&Aring;" />
 
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'''X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN'''<br />
 
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==Overview==
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==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==
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Mant (2'(3')-O-(N-methylanthraniloyl)) labeled nucleotides have proven to, be useful tools in the study of the kinetic mechanism of the myosin ATPase, by fluorescence spectroscopy. The sensitivity of the mant fluorophore to, its local environment also makes it suitable to investigate the exposure, of bound nucleotides to solvent from collisional quenching measurements., Here we present the crystal structure of mant-ADP and beryllium fluoride, complexed with Dictyostelium discoideum myosin motor domain (S1dC) at 1.9, A resolution. We complement the structural approach with an investigation, of the accessibility of the mant moiety to solvent using acrylamide, quenching of fluorescence emission. In contrast to rabbit skeletal myosin, subfragment 1, where the mant group is protected from acrylamide (Ksv=0.2, M-1), the fluorophore is relatively exposed when bound to Dictyostelium, myosin motor domain (Ksv= 1.4 M-1). Differences between the Dictyostelium, structure and that of vertebrate skeletal subfragment 1, in the region of, the nucleotide binding pocket, are proposed as an explanation for the, differences observed in the solvent accessibility of complexed, mant-nucleotides. We conclude that protection of the mant group from, acrylamide quenching does not report on overall closure of the nucleotide, binding pocket but reflects more local structural changes.
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<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [https://pdbe.org/1lvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [https://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LVK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] with MG, MNT and BEF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA].
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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X-ray crystal structure and solution fluorescence characterization of Mg.2'(3')-O-(N-methylanthraniloyl) nucleotides bound to the Dictyostelium discoideum myosin motor domain., Bauer CB, Kuhlman PA, Bagshaw CR, Rayment I, J Mol Biol. 1997 Dec 5;274(3):394-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405148 9405148]
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[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bagshaw, C.R.]]
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[[Category: Bagshaw CR]]
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[[Category: Bauer, C.B.]]
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[[Category: Bauer CB]]
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[[Category: Kuhlman, P.A.]]
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[[Category: Kuhlman PA]]
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[[Category: Rayment, I.]]
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[[Category: Rayment I]]
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[[Category: BEF]]
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[[Category: MG]]
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[[Category: MNT]]
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[[Category: actin-binding]]
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[[Category: atpase]]
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[[Category: coiled coil]]
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[[Category: dictyostelium]]
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[[Category: mant]]
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[[Category: motor]]
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[[Category: myosin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:55:20 2007''
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Current revision

X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN

PDB ID 1lvk

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