This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1m0t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:38, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1m0t.jpg|left|200px]]
 
-
{{Structure
+
==Yeast Glutathione Synthase==
-
|PDB= 1m0t |SIZE=350|CAPTION= <scene name='initialview01'>1m0t</scene>, resolution 2.30&Aring;
+
<StructureSection load='1m0t' size='340' side='right'caption='[[1m0t]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[1m0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M0T FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
|GENE= GSH2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m0t OCA], [https://pdbe.org/1m0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m0t RCSB], [https://www.ebi.ac.uk/pdbsum/1m0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m0t ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1m0t TOPSAN]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GSHB_YEAST GSHB_YEAST]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m0/1m0t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m0t ConSurf].
 +
<div style="clear:both"></div>
-
'''Yeast Glutathione Synthase'''
+
==See Also==
-
 
+
*[[Glutathione synthetase|Glutathione synthetase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Glutathione synthase catalyzes the final ATP-dependent step in glutathione biosynthesis, the formation of glutathione from gamma-glutamylcysteine and glycine. We have determined structures of yeast glutathione synthase in two forms: unbound (2.3 A resolution) and bound to its substrate gamma-glutamylcysteine, the ATP analog AMP-PNP, and two magnesium ions (1.8 A resolution). These structures reveal that upon substrate binding, large domain motions convert the enzyme from an open unliganded form to a closed conformation in which protein domains completely surround the substrate in the active site.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1M0T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0T OCA].
+
-
 
+
-
==Reference==
+
-
Large conformational changes in the catalytic cycle of glutathione synthase., Gogos A, Shapiro L, Structure. 2002 Dec;10(12):1669-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12467574 12467574]
+
-
[[Category: Glutathione synthase]]
+
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Burley SK]]
-
[[Category: Burley, S K.]]
+
[[Category: Gogos A]]
-
[[Category: Gogos, A.]]
+
[[Category: Shapiro L]]
-
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
+
-
[[Category: Shapiro, L.]]
+
-
[[Category: SO4]]
+
-
[[Category: amine/carboxylate ligase]]
+
-
[[Category: new york structural genomix research consortium]]
+
-
[[Category: nysgxrc]]
+
-
[[Category: protein structure initiative]]
+
-
[[Category: psi]]
+
-
[[Category: structural genomic]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:37:32 2008''
+

Current revision

Yeast Glutathione Synthase

PDB ID 1m0t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m0t"
Personal tools