1m1c

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(New page: 200px<br /><applet load="1m1c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m1c, resolution 3.5&Aring;" /> '''Structure of the L-A ...)
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[[Image:1m1c.jpg|left|200px]]<br /><applet load="1m1c" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m1c, resolution 3.5&Aring;" />
 
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'''Structure of the L-A virus'''<br />
 
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==Overview==
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==Structure of the L-A virus==
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The structure of the yeast L-A virus was determined by X-ray, crystallography at 3.4 A resolution. The L-A dsRNA virus is 400 A in, diameter and contains a single protein shell of 60 asymmetric dimers of, the coat protein, a feature common among the inner protein shells of dsRNA, viruses and probably related to their unique mode of transcription and, replication. The two identical subunits in each dimer are in, non-equivalent environments and show substantially different conformations, in specific surface regions. The L-A virus decaps cellular mRNA to, efficiently translate its own uncapped mRNA. Our structure reveals a, trench at the active site of the decapping reaction and suggests a role, for nearby residues in the reaction.
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<StructureSection load='1m1c' size='340' side='right'caption='[[1m1c]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m1c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_virus_L-A Saccharomyces cerevisiae virus L-A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M1C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m1c OCA], [https://pdbe.org/1m1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m1c RCSB], [https://www.ebi.ac.uk/pdbsum/1m1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m1c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAG_SCVLA GAG_SCVLA] Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 40 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm.<ref>PMID:1630453</ref> <ref>PMID:7739557</ref> Binds and removes 5' cap structures from cellular mRNA. Forms a covalent bond with m7GMP through His-154 of the capsid protein while releasing the mRNA body.<ref>PMID:1630453</ref> <ref>PMID:7739557</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m1/1m1c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m1c ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M1C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_virus_l-a Saccharomyces cerevisiae virus l-a]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M1C OCA].
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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==Reference==
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<references/>
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L-A virus at 3.4 A resolution reveals particle architecture and mRNA decapping mechanism., Naitow H, Tang J, Canady M, Wickner RB, Johnson JE, Nat Struct Biol. 2002 Oct;9(10):725-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12244300 12244300]
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__TOC__
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[[Category: Saccharomyces cerevisiae virus l-a]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Canady, M.]]
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[[Category: Saccharomyces cerevisiae virus L-A]]
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[[Category: Johnson, J.E.]]
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[[Category: Canady M]]
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[[Category: Naitow, H.]]
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[[Category: Johnson JE]]
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[[Category: Tang, J.]]
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[[Category: Naitow H]]
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[[Category: Wickner, R.B.]]
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[[Category: Tang J]]
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[[Category: dsrna virus structure; rna-protein interaction; mrna decapping; l-a virus; quai-equivalence]]
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[[Category: Wickner RB]]
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[[Category: icosahedral virus]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:03:31 2007''
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Current revision

Structure of the L-A virus

PDB ID 1m1c

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