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1m4x

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(New page: 200px<br /><applet load="1m4x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4x" /> '''PBCV-1 virus capsid, quasi-atomic model'''<b...)
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[[Image:1m4x.gif|left|200px]]<br /><applet load="1m4x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m4x" />
 
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'''PBCV-1 virus capsid, quasi-atomic model'''<br />
 
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==Overview==
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==PBCV-1 virus capsid, quasi-atomic model==
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Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a, glycoprotein coat consisting of pseudohexagonal arrays of trimeric, capsomers. Each capsomer is composed of three molecules of the major, capsid protein, Vp54, the 2.0-A resolution structure of which is reported, here. Four N-linked and two O-linked glycosylation sites were identified., The N-linked sites are associated with nonstandard amino acid motifs as a, result of glycosylation by virus-encoded enzymes. Each monomer of the, trimeric structure consists of two eight-stranded, antiparallel, beta-barrel, "jelly-roll" domains related by a pseudo-sixfold rotation., The fold of the monomer and the pseudo-sixfold symmetry of the capsomer, resembles that of the major coat proteins in the double-stranded DNA, bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well, as the viral proteins VP2-VP3 of picornaviruses. The structural, similarities among these diverse groups of viruses, whose hosts include, bacteria, unicellular eukaryotes, plants, and mammals, make it probable, that their capsid proteins have evolved from a common ancestor that had, already acquired a pseudo-sixfold organization. The trimeric capsid, protein structure was used to produce a quasi-atomic model of the 1,900-A, diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal, structure into a three-dimensional cryoelectron microscopy image, reconstruction of the virus.
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<SX load='1m4x' size='340' side='right' viewer='molstar' caption='[[1m4x]], [[Resolution|resolution]] 28.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m4x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M4X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 28&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m4x OCA], [https://pdbe.org/1m4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m4x RCSB], [https://www.ebi.ac.uk/pdbsum/1m4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m4x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCP_PBCV1 MCP_PBCV1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/1m4x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m4x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M4X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_type_1_(pbcv-1) Paramecium bursaria chlorella virus type 1 (pbcv-1)]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M4X OCA].
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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__TOC__
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==Reference==
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</SX>
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The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus., Nandhagopal N, Simpson AA, Gurnon JR, Yan X, Baker TS, Graves MV, Van Etten JL, Rossmann MG, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14758-63. Epub 2002 Oct 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12411581 12411581]
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[[Category: Large Structures]]
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[[Category: Paramecium bursaria chlorella virus type 1 (pbcv-1)]]
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[[Category: Paramecium bursaria Chlorella virus 1]]
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[[Category: Single protein]]
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[[Category: Baker TS]]
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[[Category: Baker, T.S.]]
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[[Category: Graves MV]]
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[[Category: Etten, J.L.Van.]]
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[[Category: Gurnon JR]]
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[[Category: Graves, M.V.]]
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[[Category: Nandhagopal N]]
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[[Category: Gurnon, J.R.]]
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[[Category: Rossmann MG]]
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[[Category: Nandhagopal, N.]]
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[[Category: Simpson AA]]
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[[Category: Rossmann, M.G.]]
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[[Category: Van Etten JL]]
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[[Category: Simpson, A.A.]]
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[[Category: Yan X]]
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[[Category: Yan, X.]]
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[[Category: beta barrel]]
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[[Category: icosahedral virus]]
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[[Category: icosahedral virus capsid]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:08:57 2007''
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Current revision

PBCV-1 virus capsid, quasi-atomic model

1m4x, resolution 28.00Å

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