1m6j

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[[Image:1m6j.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ENTAMOEBA HISTOLYTICA==
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The line below this paragraph, containing "STRUCTURE_1m6j", creates the "Structure Box" on the page.
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<StructureSection load='1m6j' size='340' side='right'caption='[[1m6j]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1m6j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M6J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m6j OCA], [https://pdbe.org/1m6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m6j RCSB], [https://www.ebi.ac.uk/pdbsum/1m6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m6j ProSAT]</span></td></tr>
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{{STRUCTURE_1m6j| PDB=1m6j | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPIS_ENTHI TPIS_ENTHI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m6/1m6j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m6j ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ENTAMOEBA HISTOLYTICA'''
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==See Also==
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*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Triosephosphate isomerase (TIM) has been proposed as a target for drug design. TIMs from several parasites have a cysteine residue at the dimer interface, whose derivatization with thiol-specific reagents induces enzyme inactivation and aggregation. TIMs lacking this residue, such as human TIM, are less affected. TIM from Entamoeba histolytica (EhTIM) has the interface cysteine residue and presents more than ten insertions when compared with the enzyme from other pathogens. To gain further insight into the role that interface residues play in the stability and reactivity of these enzymes, we determined the high-resolution structure and characterized the effect of methylmethane thiosulfonate (MMTS) on the activity and conformational properties of EhTIM. The structure of this enzyme was determined at 1.5A resolution using molecular replacement, observing that the dimer is not symmetric. EhTIM is completely inactivated by MMTS, and dissociated into stable monomers that possess considerable secondary structure. Structural and spectroscopic analysis of EhTIM and comparison with TIMs from other pathogens reveal that conformational rearrangements of the interface after dissociation, as well as intramonomeric contacts formed by the inserted residues, may contribute to the unusual stability of the derivatized EhTIM monomer.
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==About this Structure==
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1M6J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6J OCA].
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==Reference==
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Structure and inactivation of triosephosphate isomerase from Entamoeba histolytica., Rodriguez-Romero A, Hernandez-Santoyo A, del Pozo Yauner L, Kornhauser A, Fernandez-Velasco DA, J Mol Biol. 2002 Sep 27;322(4):669-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12270704 12270704]
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[[Category: Entamoeba histolytica]]
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[[Category: Single protein]]
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[[Category: Triose-phosphate isomerase]]
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[[Category: Fernandez-Velasco, D A.]]
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[[Category: Hernandez-Santoyo, A.]]
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[[Category: Rodriguez-Romero, A.]]
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[[Category: Asymmetry]]
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[[Category: Entamoeba histolytica]]
[[Category: Entamoeba histolytica]]
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[[Category: Monomer stability]]
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[[Category: Large Structures]]
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[[Category: Triosephosphate isomerase]]
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[[Category: Fernandez-Velasco DA]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:41:46 2008''
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[[Category: Hernandez-Santoyo A]]
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[[Category: Rodriguez-Romero A]]

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CRYSTAL STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ENTAMOEBA HISTOLYTICA

PDB ID 1m6j

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