1m6u

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[[Image:1m6u.jpg|left|200px]]<br /><applet load="1m6u" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m6u, resolution 2.3&Aring;" />
 
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'''Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast'''<br />
 
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==Overview==
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==Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast==
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Ndt80 is a transcriptional activator required for meiosis in the yeast Saccharomyces cerevisiae. Here, we report the crystal structure at 2.3 A resolution of the DNA-binding domain of Ndt80 experimentally phased by using the anomalous and isomorphous signal from a single ordered Se atom per molecule of 272-aa residues. The structure reveals a single approximately 32-kDa domain with a distinct fold comprising a beta-sandwich core elaborated with seven additional beta-sheets and three short alpha-helices. Inspired by the structure, we have performed a mutational analysis and defined a DNA-binding motif in this domain. The DNA-binding domain of Ndt80 is homologous to a number of proteins from higher eukaryotes, and the residues that we have shown are required for DNA binding by Ndt80 are highly conserved among this group of proteins. These results suggest that Ndt80 is the defining member of a previously uncharacterized family of transcription factors, including the human protein (C11orf9), which has been shown to be highly expressed in invasive or metastatic tumor cells.
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<StructureSection load='1m6u' size='340' side='right'caption='[[1m6u]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m6u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M6U FirstGlance]. <br>
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1M6U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m6u OCA], [https://pdbe.org/1m6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m6u RCSB], [https://www.ebi.ac.uk/pdbsum/1m6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m6u ProSAT]</span></td></tr>
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Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast., Montano SP, Cote ML, Fingerman I, Pierce M, Vershon AK, Georgiadis MM, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14041-6. Epub 2002 Oct 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12384578 12384578]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NDT80_YEAST NDT80_YEAST] Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.<ref>PMID:8524222</ref> <ref>PMID:9660952</ref> <ref>PMID:9742092</ref> <ref>PMID:12832469</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m6/1m6u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m6u ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Cote ML]]
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[[Category: Cote, M L.]]
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[[Category: Fingerman I]]
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[[Category: Fingerman, I.]]
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[[Category: Georgiadis MM]]
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[[Category: Georgiadis, M M.]]
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[[Category: Montano SP]]
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[[Category: Montano, S P.]]
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[[Category: Pierce M]]
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[[Category: Pierce, M.]]
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[[Category: Vershon AK]]
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[[Category: Vershon, A K.]]
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[[Category: SO4]]
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[[Category: dna-binding]]
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[[Category: meiosis]]
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[[Category: transcription activator]]
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[[Category: yeast protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:07 2008''
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Current revision

Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast

PDB ID 1m6u

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