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1m7w

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(New page: 200px<br /><applet load="1m7w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m7w, resolution 2.8&Aring;" /> '''HNF4a ligand binding ...)
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[[Image:1m7w.gif|left|200px]]<br /><applet load="1m7w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m7w, resolution 2.8&Aring;" />
 
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'''HNF4a ligand binding domain with bound fatty acid'''<br />
 
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==Overview==
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==HNF4a ligand binding domain with bound fatty acid==
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HNF4 alpha is an orphan member of the nuclear receptor family with, prominent functions in liver, gut, kidney and pancreatic beta cells. We, have solved the x-ray crystal structure of the HNF4 alpha ligand binding, domain, which adopts a canonical fold. Two conformational states are, present within each homodimer: an open form with alpha helix 12 (alpha 12), extended and collinear with alpha 10 and a closed form with alpha 12, folded against the body of the domain. Although the protein was, crystallized without added ligands, the ligand binding pockets of both, closed and open forms contain fatty acids. The carboxylic acid headgroup, of the fatty acid ion pairs with the guanidinium group of Arg(226) at one, end of the ligand binding pocket, while the aliphatic chain fills a long, narrow channel that is lined with hydrophobic residues. These findings, suggest that fatty acids are endogenous ligands for HNF4 alpha and, establish a framework for understanding how HNF4 alpha activity is, enhanced by ligand binding and diminished by MODY1 mutations.
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<StructureSection load='1m7w' size='340' side='right'caption='[[1m7w]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m7w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7W FirstGlance]. <br>
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1M7W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with DAO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M7W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7w OCA], [https://pdbe.org/1m7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7w RCSB], [https://www.ebi.ac.uk/pdbsum/1m7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7w ProSAT]</span></td></tr>
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Crystal structure of the HNF4 alpha ligand binding domain in complex with endogenous fatty acid ligand., Dhe-Paganon S, Duda K, Iwamoto M, Chi YI, Shoelson SE, J Biol Chem. 2002 Oct 11;277(41):37973-6. Epub 2002 Aug 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12193589 12193589]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HNF4A_RAT HNF4A_RAT] Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7w ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus rattus]]
[[Category: Rattus rattus]]
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[[Category: Single protein]]
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[[Category: Chi YI]]
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[[Category: Chi, Y.I.]]
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[[Category: Dhe-Paganon S]]
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[[Category: Dhe-Paganon, S.]]
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[[Category: Duda K]]
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[[Category: Duda, K.]]
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[[Category: Iwamoto M]]
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[[Category: Iwamoto, M.]]
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[[Category: Shoelson SE]]
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[[Category: Shoelson, S.E.]]
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[[Category: DAO]]
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[[Category: transcription factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:12:54 2007''
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Current revision

HNF4a ligand binding domain with bound fatty acid

PDB ID 1m7w

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