1m93

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:41, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1m93.gif|left|200px]]
 
-
{{Structure
+
==1.65 A Structure of Cleaved Viral Serpin CRMA==
-
|PDB= 1m93 |SIZE=350|CAPTION= <scene name='initialview01'>1m93</scene>, resolution 1.65&Aring;
+
<StructureSection load='1m93' size='340' side='right'caption='[[1m93]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+
<table><tr><td colspan='2'>[[1m93]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M93 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-
|GENE= crmA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10243 Cowpox virus])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m93 OCA], [https://pdbe.org/1m93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m93 RCSB], [https://www.ebi.ac.uk/pdbsum/1m93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m93 ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1c8o|1C8O]], [[1f0c|1F0C]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m93 OCA], [http://www.ebi.ac.uk/pdbsum/1m93 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m93 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m93_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m93 ConSurf].
 +
<div style="clear:both"></div>
-
'''1.65 A Structure of Cleaved Viral Serpin CRMA'''
+
==See Also==
-
 
+
*[[Serpin 3D structures|Serpin 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1M93 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M93 OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition., Simonovic M, Gettins PGW, Volz K, Protein Sci. 2000 Aug;9(8):1423-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10975564 10975564]
+
[[Category: Cowpox virus]]
[[Category: Cowpox virus]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Gettins, P G.W.]]
+
[[Category: Gettins PGW]]
-
[[Category: Simonovic, M.]]
+
[[Category: Simonovic M]]
-
[[Category: Volz, K.]]
+
[[Category: Volz K]]
-
[[Category: apoptosis]]
+
-
[[Category: crma]]
+
-
[[Category: ice inhibitor]]
+
-
[[Category: serpin]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:04 2008''
+

Current revision

1.65 A Structure of Cleaved Viral Serpin CRMA

PDB ID 1m93

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m93"
Personal tools