1mby

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[[Image:1mby.jpg|left|200px]]
 
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==Murine Sak Polo Domain==
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The line below this paragraph, containing "STRUCTURE_1mby", creates the "Structure Box" on the page.
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<StructureSection load='1mby' size='340' side='right'caption='[[1mby]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MBY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mby OCA], [https://pdbe.org/1mby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mby RCSB], [https://www.ebi.ac.uk/pdbsum/1mby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mby ProSAT]</span></td></tr>
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{{STRUCTURE_1mby| PDB=1mby | SCENE= }}
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</table>
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== Function ==
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'''Murine Sak Polo Domain'''
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[https://www.uniprot.org/uniprot/PLK4_MOUSE PLK4_MOUSE] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2 (By similarity). Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1.<ref>PMID:17891141</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The small family of polo-like kinases (Plks) includes Cdc5 from Saccharomyces cerevisiae, Plo1 from Schizosaccharomyces pombe, Polo from Drosophila melanogaster and the four mammalian genes Plk1, Prk/Fnk, Snk and Sak. These kinases control cell cycle progression through the regulation of centrosome maturation and separation, mitotic entry, metaphase to anaphase transition, mitotic exit and cytokinesis. Plks are characterized by an N-terminal Ser/Thr protein kinase domain and the presence of one or two C-terminal regions of similarity, termed the polo box motifs. These motifs have been demonstrated for Cdc5 and Plk1 to be required for mitotic progression and for subcellular localization to mitotic structures. Here we report the 2.0 A crystal structure of a novel domain composed of the polo box motif of murine Sak. The structure consists of a dimeric fold with a deep interfacial cleft and pocket, suggestive of a ligand-binding site. We show that this domain forms homodimers both in vitro and in vivo, and localizes to centrosomes and the cleavage furrow during cytokinesis. The requirement of the polo domain for Plk family function and the unique physical properties of the domain identify it as an attractive target for inhibitor design.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mby_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1MBY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBY OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mby ConSurf].
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The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization., Leung GC, Hudson JW, Kozarova A, Davidson A, Dennis JW, Sicheri F, Nat Struct Biol. 2002 Oct;9(10):719-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12352953 12352953]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Davidson A]]
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[[Category: Single protein]]
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[[Category: Dennis JW]]
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[[Category: Davidson, A.]]
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[[Category: Hudson JW]]
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[[Category: Dennis, J W.]]
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[[Category: Kozarova A]]
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[[Category: Hudson, J W.]]
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[[Category: Leung GC]]
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[[Category: Kozarova, A.]]
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[[Category: Sicheri F]]
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[[Category: Leung, G C.]]
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[[Category: Sicheri, F.]]
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[[Category: Polo domain]]
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[[Category: Strand exchange]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:52:16 2008''
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Current revision

Murine Sak Polo Domain

PDB ID 1mby

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