1mby

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1mby" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mby, resolution 2.0&Aring;" /> '''Murine Sak Polo Domai...)
Current revision (07:43, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mby.jpg|left|200px]]<br /><applet load="1mby" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1mby, resolution 2.0&Aring;" />
 
-
'''Murine Sak Polo Domain'''<br />
 
-
==Overview==
+
==Murine Sak Polo Domain==
-
The small family of polo-like kinases (Plks) includes Cdc5 from, Saccharomyces cerevisiae, Plo1 from Schizosaccharomyces pombe, Polo from, Drosophila melanogaster and the four mammalian genes Plk1, Prk/Fnk, Snk, and Sak. These kinases control cell cycle progression through the, regulation of centrosome maturation and separation, mitotic entry, metaphase to anaphase transition, mitotic exit and cytokinesis. Plks are, characterized by an N-terminal Ser/Thr protein kinase domain and the, presence of one or two C-terminal regions of similarity, termed the polo, box motifs. These motifs have been demonstrated for Cdc5 and Plk1 to be, required for mitotic progression and for subcellular localization to, mitotic structures. Here we report the 2.0 A crystal structure of a novel, domain composed of the polo box motif of murine Sak. The structure, consists of a dimeric fold with a deep interfacial cleft and pocket, suggestive of a ligand-binding site. We show that this domain forms, homodimers both in vitro and in vivo, and localizes to centrosomes and the, cleavage furrow during cytokinesis. The requirement of the polo domain for, Plk family function and the unique physical properties of the domain, identify it as an attractive target for inhibitor design.
+
<StructureSection load='1mby' size='340' side='right'caption='[[1mby]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1mby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MBY FirstGlance]. <br>
-
1MBY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MBY OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mby OCA], [https://pdbe.org/1mby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mby RCSB], [https://www.ebi.ac.uk/pdbsum/1mby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mby ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization., Leung GC, Hudson JW, Kozarova A, Davidson A, Dennis JW, Sicheri F, Nat Struct Biol. 2002 Oct;9(10):719-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12352953 12352953]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/PLK4_MOUSE PLK4_MOUSE] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2 (By similarity). Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1.<ref>PMID:17891141</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mby_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mby ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Non-specific serine/threonine protein kinase]]
+
[[Category: Davidson A]]
-
[[Category: Single protein]]
+
[[Category: Dennis JW]]
-
[[Category: Davidson, A.]]
+
[[Category: Hudson JW]]
-
[[Category: Dennis, J.W.]]
+
[[Category: Kozarova A]]
-
[[Category: Hudson, J.W.]]
+
[[Category: Leung GC]]
-
[[Category: Kozarova, A.]]
+
[[Category: Sicheri F]]
-
[[Category: Leung, G.C.]]
+
-
[[Category: Sicheri, F.]]
+
-
[[Category: polo domain]]
+
-
[[Category: strand exchange]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:19:12 2007''
+

Current revision

Murine Sak Polo Domain

PDB ID 1mby

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mby"
Personal tools