This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1mbz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1mbz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mbz, resolution 2.47&Aring;" /> '''BETA-LACTAM SYNTHETA...)
Current revision (07:43, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mbz.gif|left|200px]]<br /><applet load="1mbz" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1mbz, resolution 2.47&Aring;" />
 
-
'''BETA-LACTAM SYNTHETASE WITH TRAPPED INTERMEDIATE'''<br />
 
-
==Overview==
+
==BETA-LACTAM SYNTHETASE WITH TRAPPED INTERMEDIATE==
-
The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam, synthetase (beta-LS) from Streptomyces clavuligerus has been observed, through a series of x-ray crystallographic snapshots. Chemistry is, initiated by the ordered binding of ATP/Mg(2+) and, N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and, ATP/Mg(2+) structures described here, along with the previously described, CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+), structure, illuminate changes in active site geometry that favor, adenylation. In addition, an acyladenylate intermediate has been trapped., The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated, by ATP in the crystal and represents a close structural analog of the, previously proposed CEA-adenylate intermediate. Finally, the structure of, the ternary product complex deoxyguanidinoproclavaminic acid, (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and, DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site, that facilitate beta-lactam formation. All of the ATP-bound structures, differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that, two Mg(2+) ions are found in the active sites. These Mg(2+) ions play, critical roles in both the adenylation and beta-lactamization reactions.
+
<StructureSection load='1mbz' size='340' side='right'caption='[[1mbz]], [[Resolution|resolution]] 2.47&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1mbz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MBZ FirstGlance]. <br>
-
1MBZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with MG, POP, IOT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MBZ OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.47&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOT:ARGININE-N-METHYLCARBONYL+PHOSPHORIC+ACID+5-ADENOSINE+ESTER'>IOT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mbz OCA], [https://pdbe.org/1mbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mbz RCSB], [https://www.ebi.ac.uk/pdbsum/1mbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mbz ProSAT]</span></td></tr>
-
The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12409610 12409610]
+
</table>
-
[[Category: Single protein]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/BLS_STRCL BLS_STRCL]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mbz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mbz ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
-
[[Category: Bachmann, B.O.]]
+
[[Category: Bachmann BO]]
-
[[Category: Miller, M.T.]]
+
[[Category: Miller MT]]
-
[[Category: Rosenzweig, A.C.]]
+
[[Category: Rosenzweig AC]]
-
[[Category: Townsend, C.A.]]
+
[[Category: Townsend CA]]
-
[[Category: GOL]]
+
-
[[Category: IOT]]
+
-
[[Category: MG]]
+
-
[[Category: POP]]
+
-
[[Category: asparagine synthetase]]
+
-
[[Category: beta-lactam synthetase]]
+
-
[[Category: carboxyethyl arginine]]
+
-
[[Category: clavulanic acid]]
+
-
[[Category: deoxyguanidinoproclavaminic acid]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:19:15 2007''
+

Current revision

BETA-LACTAM SYNTHETASE WITH TRAPPED INTERMEDIATE

PDB ID 1mbz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mbz"
Personal tools