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1mee

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(New page: 200px<br /><applet load="1mee" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mee, resolution 2.0&Aring;" /> '''THE COMPLEX BETWEEN T...)
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[[Image:1mee.gif|left|200px]]<br /><applet load="1mee" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mee, resolution 2.0&Aring;" />
 
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'''THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C'''<br />
 
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==Overview==
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==THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C==
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The alkaline proteinase from the mesophilic bacterium Bacillus, mesentericus has been crystallized in a 1:1 complex with the inhibitor, eglin-C from the medical leech. The crystals have cell dimensions of a =, 43.0, b = 71.9, c = 48.3 A and beta = 110.0 degrees and are in the space, group P2(1). Three-dimensional data to 2.0 A have been recorded on film, from a single crystal. The orientation and position of the complex in the, unit cell have been established using the refined coordinates of, subtilisin Carlsberg and of eglin-C as independent models. The structure, of the complex has been refined by restrained least-squares minimization., The crystallographic R factor (= sigma[magnitude of Fo - magnitude of, Fc[/sigma magnitude of Fo) is 15.1% including two Ca2+ ions and 312 water, molecules. The structure is discussed in terms of its physicochemical, properties in solution and its relation to other Bacillus subtilisins.
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<StructureSection load='1mee' size='340' side='right'caption='[[1mee]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mee]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_pumilus Bacillus pumilus] and [https://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mee OCA], [https://pdbe.org/1mee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mee RCSB], [https://www.ebi.ac.uk/pdbsum/1mee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mee ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SUBT_BACPU SUBT_BACPU] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mee_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mee ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MEE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_pumilus Bacillus pumilus] and [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEE OCA].
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*[[Eglin|Eglin]]
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*[[Subtilisin 3D structures|Subtilisin 3D structures]]
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==Reference==
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__TOC__
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Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C., Dauter Z, Betzel C, Genov N, Pipon N, Wilson KS, Acta Crystallogr B. 1991 Oct 1;47 ( Pt 5):707-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1793542 1793542]
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</StructureSection>
[[Category: Bacillus pumilus]]
[[Category: Bacillus pumilus]]
[[Category: Hirudo medicinalis]]
[[Category: Hirudo medicinalis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Subtilisin]]
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[[Category: Betzel C]]
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[[Category: Betzel, C.]]
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[[Category: Dauter Z]]
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[[Category: Dauter, Z.]]
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[[Category: Wilson KS]]
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[[Category: Wilson, K.S.]]
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[[Category: CA]]
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[[Category: complex(serine proteinase-inhibitor)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:22:11 2007''
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Current revision

THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C

PDB ID 1mee

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