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1mg5

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(New page: 200px<br /><applet load="1mg5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mg5, resolution 1.63&Aring;" /> '''Crystal structure of...)
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[[Image:1mg5.gif|left|200px]]<br /><applet load="1mg5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mg5, resolution 1.63&Aring;" />
 
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'''Crystal structure of Drosophila melanogaster alcohol dehydrogenase complexed with NADH and acetate at 1.6 A'''<br />
 
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==Overview==
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==Crystal structure of Drosophila melanogaster alcohol dehydrogenase complexed with NADH and acetate at 1.6 A==
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Drosophila alcohol dehydrogenase (DADH) is an NAD+-dependent enzyme that, catalyzes the oxidation of alcohols to aldehydes/ketones and that is also, able to further oxidize aldehydes to their corresponding carboxylic acids., The structure of the ternary enzyme-NADH-acetate complex of the slow, alleloform of Drosophila melanogaster ADH (DmADH-S) was solved at 1.6 A, resolution by X-ray crystallography. The coenzyme stereochemistry of the, aldehyde dismutation reaction showed that the obtained enzyme-NADH-acetate, complex reflects a productive ternary complex although no enzymatic, reaction occurs. The stereochemistry of the acetate binding in the, bifurcated substrate-binding site, along with previous stereochemical, studies of aldehyde reduction and alcohol oxidation shows that the methyl, group of the aldehyde in the reduction reaction binds to the R1 and in the, oxidation reaction to the R2 sub-site. NMR studies along with previous, kinetic studies show that the formed acetaldehyde intermediate in the, oxidation of ethanol to acetate leaves the substrate site prior to the, reduced coenzyme, and then binds to the newly formed enzyme-NAD+ complex., Here, we compare the three-dimensional structure of D.melanogaster ADH-S, and a previous theoretically built model, evaluate the differences with, the crystal structures of five Drosophila lebanonensis ADHs in numerous, complexed forms that explain the substrate specificity as well as subtle, kinetic differences between these two enzymes based on their crystal, structures. We also re-examine the electrostatic influence of charged, residues on the surface of the protein on the catalytic efficiency of the, enzyme.
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<StructureSection load='1mg5' size='340' side='right'caption='[[1mg5]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mg5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MG5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mg5 OCA], [https://pdbe.org/1mg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1mg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mg5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ADH_DROME ADH_DROME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mg5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mg5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ACT and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MG5 OCA].
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Drosophila alcohol dehydrogenase: acetate-enzyme interactions and novel insights into the effects of electrostatics on catalysis., Benach J, Winberg JO, Svendsen JS, Atrian S, Gonzalez-Duarte R, Ladenstein R, J Mol Biol. 2005 Jan 21;345(3):579-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15581900 15581900]
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[[Category: Alcohol dehydrogenase]]
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Atrian, S.]]
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[[Category: Atrian S]]
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[[Category: Benach, J.]]
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[[Category: Benach J]]
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[[Category: Gonzalez-Duarte, R.]]
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[[Category: Gonzalez-Duarte R]]
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[[Category: Ladenstein, R.]]
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[[Category: Ladenstein R]]
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[[Category: ACT]]
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[[Category: NAD]]
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[[Category: acetate]]
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[[Category: adh]]
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[[Category: drosophila melanogaster]]
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[[Category: nadh]]
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[[Category: sdr]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:23:48 2007''
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Current revision

Crystal structure of Drosophila melanogaster alcohol dehydrogenase complexed with NADH and acetate at 1.6 A

PDB ID 1mg5

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