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1mhs

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[[Image:1mhs.gif|left|200px]]<br /><applet load="1mhs" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mhs, resolution 8.0&Aring;" />
 
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'''Model of Neurospora crassa proton ATPase'''<br />
 
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==Overview==
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==Model of Neurospora crassa proton ATPase==
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Proton pumps in the plasma membrane of plants and yeasts maintain the intracellular pH and membrane potential. To gain insight into the molecular mechanisms of proton pumping, we built an atomic homology model of the proton pump based on the 2.6 angstrom x-ray structure of the related Ca2+ pump from rabbit sarcoplasmic reticulum. The model, when fitted to an 8 angstrom map of the Neurospora proton pump determined by electron microscopy, reveals the likely path of the proton through the membrane and shows that the nucleotide-binding domain rotates by approximately 70 degrees to deliver adenosine triphosphate (ATP) to the phosphorylation site. A synthetic peptide corresponding to the carboxyl-terminal regulatory domain stimulates ATPase activity, suggesting a mechanism for proton transport regulation.
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<StructureSection load='1mhs' size='340' side='right'caption='[[1mhs]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mhs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MHS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mhs OCA], [https://pdbe.org/1mhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mhs RCSB], [https://www.ebi.ac.uk/pdbsum/1mhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mhs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PMA1_NEUCR PMA1_NEUCR] The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/1mhs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mhs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MHS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Active as [http://en.wikipedia.org/wiki/Proton-exporting_ATPase Proton-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.6 3.6.3.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHS OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure, mechanism, and regulation of the Neurospora plasma membrane H+-ATPase., Kuhlbrandt W, Zeelen J, Dietrich J, Science. 2002 Sep 6;297(5587):1692-6. Epub 2002 Aug 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12169656 12169656]
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[[Category: Large Structures]]
[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Proton-exporting ATPase]]
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[[Category: Kuhlbrandt W]]
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[[Category: Single protein]]
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[[Category: Kuhlbrandt, W.]]
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[[Category: active transport]]
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[[Category: cryo-electron microscopy]]
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[[Category: ion transport]]
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[[Category: membrane protein]]
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[[Category: p-type atpase]]
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[[Category: proton pump]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:18 2008''
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Current revision

Model of Neurospora crassa proton ATPase

PDB ID 1mhs

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