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1mhx

From Proteopedia

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[[Image:1mhx.jpg|left|200px]]<br /><applet load="1mhx" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mhx, resolution 1.8&Aring;" />
 
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'''Crystal Structures of the redesigned protein G variant NuG1'''<br />
 
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==Overview==
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==Crystal Structures of the redesigned protein G variant NuG1==
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We recently described two protein G variants (NuG1 and NuG2) with redesigned first hairpins that were almost twice as stable, folded 100-fold faster, and had a switched folding mechanism relative to the wild-type protein. To test the structural accuracy of our design algorithm and to provide insights to the dramatic changes in the kinetics and thermodynamics of folding, we have now determined the crystal structures of NuG1 and NuG2 to 1.8 A and 1.85 A, respectively. We find that they adopt hairpin structures that are closer to the computational models than to wild-type protein G; the RMSD of the NuG1 hairpin to the design model and the wild-type structure are 1.7 A and 5.1 A, respectively. The crystallographic B factor in the redesigned first hairpin of NuG1 is systematically higher than the second hairpin, suggesting that the redesigned region is somewhat less rigid. A second round of structure-based design yielded new variants of NuG1 and NuG2, which are further stabilized by 0.5 kcal/mole and 0.9 kcal/mole.
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<StructureSection load='1mhx' size='340' side='right'caption='[[1mhx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mhx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna_ATCC_29328 Finegoldia magna ATCC 29328]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MHX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mhx OCA], [https://pdbe.org/1mhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mhx RCSB], [https://www.ebi.ac.uk/pdbsum/1mhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mhx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q53291_FINMA Q53291_FINMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/1mhx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mhx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MHX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHX OCA].
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*[[Protein G|Protein G]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12441390 12441390]
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[[Category: Finegoldia magna ATCC 29328]]
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[[Category: Finegoldia magna]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Baker D]]
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[[Category: Baker, D.]]
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[[Category: Kuhlman B]]
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[[Category: Kuhlman, B.]]
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[[Category: Le Trong I]]
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[[Category: Nauli, S.]]
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[[Category: Nauli S]]
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[[Category: Stenkamp, R E.]]
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[[Category: Stenkamp RE]]
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[[Category: Teller, D C.]]
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[[Category: Teller DC]]
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[[Category: Trong, I Le.]]
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[[Category: alpha-beta protein]]
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[[Category: redesigned first beta-hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:24 2008''
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Current revision

Crystal Structures of the redesigned protein G variant NuG1

PDB ID 1mhx

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