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1mjt

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[[Image:1mjt.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF SANOS, A BACTERIAL NITRIC OXIDE SYNTHASE OXYGENASE PROTEIN, IN COMPLEX WITH NAD+ AND SEITU==
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The line below this paragraph, containing "STRUCTURE_1mjt", creates the "Structure Box" on the page.
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<StructureSection load='1mjt' size='340' side='right'caption='[[1mjt]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mjt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ITU:ETHYLISOTHIOUREA'>ITU</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
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{{STRUCTURE_1mjt| PDB=1mjt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjt OCA], [https://pdbe.org/1mjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjt RCSB], [https://www.ebi.ac.uk/pdbsum/1mjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjt ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF SANOS, A BACTERIAL NITRIC OXIDE SYNTHASE OXYGENASE PROTEIN, IN COMPLEX WITH NAD+ AND SEITU'''
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== Function ==
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[https://www.uniprot.org/uniprot/NOSO_STAAU NOSO_STAAU] Catalyzes the production of nitric oxide (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Prokaryotic genes related to the oxygenase domain of mammalian nitric oxide synthases (NOSs) have recently been identified. Although they catalyze the same reaction as the eukaryotic NOS oxygenase domain, their biological function(s) are unknown. In order to explore rationally the biochemistry and evolution of the prokaryotic NOS family, we have determined the crystal structure of SANOS, from methicillin-resistant Staphylococcus aureus (MRSA), to 2.4 A. Haem and S-ethylisothiourea (SEITU) are bound at the SANOS active site, while the intersubunit site, occupied by the redox cofactor tetrahydrobiopterin (H(4)B) in mammalian NOSs, has NAD(+) bound in SANOS. In common with all bacterial NOSs, SANOS lacks the N-terminal extension responsible for stable dimerization in mammalian isoforms, but has alternative interactions to promote dimer formation.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjt_consurf.spt"</scriptWhenChecked>
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1MJT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJT OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of SANOS, a bacterial nitric oxide synthase oxygenase protein from Staphylococcus aureus., Bird LE, Ren J, Zhang J, Foxwell N, Hawkins AR, Charles IG, Stammers DK, Structure. 2002 Dec;10(12):1687-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12467576 12467576]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjt ConSurf].
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[[Category: Nitric-oxide synthase]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Staphylococcus aureus]]
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</StructureSection>
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[[Category: Bird, L E.]]
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[[Category: Large Structures]]
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[[Category: Ren, J.]]
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[[Category: Stammers, D K.]]
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[[Category: Bacterial]]
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[[Category: Mrsa]]
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[[Category: No]]
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[[Category: No]]
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[[Category: Sano]]
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[[Category: Seitu]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Synthase]]
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[[Category: Bird LE]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:13:35 2008''
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[[Category: Ren J]]
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[[Category: Stammers DK]]

Current revision

CRYSTAL STRUCTURE OF SANOS, A BACTERIAL NITRIC OXIDE SYNTHASE OXYGENASE PROTEIN, IN COMPLEX WITH NAD+ AND SEITU

PDB ID 1mjt

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