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1mjw

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[[Image:1mjw.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D42N==
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|PDB= 1mjw |SIZE=350|CAPTION= <scene name='initialview01'>1mjw</scene>, resolution 1.95&Aring;
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<StructureSection load='1mjw' size='340' side='right'caption='[[1mjw]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1mjw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJW FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjw OCA], [https://pdbe.org/1mjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjw RCSB], [https://www.ebi.ac.uk/pdbsum/1mjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjw ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjw OCA], [http://www.ebi.ac.uk/pdbsum/1mjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mjw RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IPYR_ECOLI IPYR_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjw ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D42N'''
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==See Also==
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The three-dimensional structures of four mutant E. coli inorganic pyrophosphatases (PPases) with single Asp--&gt;Asn substitutions at positions 42, 65, 70, and 97 were solved at 1.95, 2.15, 2.10, and 2.20 A resolution, respectively. Asp-42--&gt;Asn and Asp-65--&gt;Asn mutant PPases were prepared as complexes with sulfate--a structural analog of phosphate, the product of enzymatic reaction. A comparison of mutant enzymes with native PPases revealed that a single amino acid substitution changes the position of the mutated residue as well as the positions of several functional groups and some parts of a polypeptide chain. These changes are responsible for the fact that mutant PPases differ from the native ones in their catalytic properties. The sulfate binding to the mutant PPase active site causes molecular asymmetry, as shown for the native PPase earlier. The subunit asymmetry is manifested in different positions of sulfate and several functional groups, as well as changes in packing of hexamers in crystals and in cell parameters.
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==About this Structure==
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1MJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJW OCA].
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==Reference==
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Three-dimensional structures of mutant forms of E. coli inorganic pyrophosphatase with Asp--&gt;Asn single substitution in positions 42, 65, 70, and 97., Avaeva SM, Rodina EV, Vorobyeva NN, Kurilova SA, Nazarova TI, Sklyankina VA, Oganessyan VY, Samygina VR, Harutyunyan EH, Biochemistry (Mosc). 1998 Jun;63(6):671-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9668207 9668207]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Avaeva SM]]
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[[Category: Avaeva, S M.]]
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[[Category: Harutyunyan EH]]
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[[Category: Harutyunyan, E H.]]
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[[Category: Huber R]]
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[[Category: Huber, R.]]
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[[Category: Oganesyan V]]
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[[Category: Oganesyan, V.]]
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[[Category: Samygina VR]]
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[[Category: Samygina, V R.]]
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[[Category: acid anhydride hydrolase]]
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[[Category: hydrolase]]
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[[Category: mutation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:58 2008''
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Current revision

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D42N

PDB ID 1mjw

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