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1mjx

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[[Image:1mjx.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D65N==
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|PDB= 1mjx |SIZE=350|CAPTION= <scene name='initialview01'>1mjx</scene>, resolution 2.15&Aring;
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<StructureSection load='1mjx' size='340' side='right'caption='[[1mjx]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1mjx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJX FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjx OCA], [https://pdbe.org/1mjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjx RCSB], [https://www.ebi.ac.uk/pdbsum/1mjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPYR_ECOLI IPYR_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjx ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D65N'''
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==See Also==
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*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The three-dimensional structures of four mutant E. coli inorganic pyrophosphatases (PPases) with single Asp--&gt;Asn substitutions at positions 42, 65, 70, and 97 were solved at 1.95, 2.15, 2.10, and 2.20 A resolution, respectively. Asp-42--&gt;Asn and Asp-65--&gt;Asn mutant PPases were prepared as complexes with sulfate--a structural analog of phosphate, the product of enzymatic reaction. A comparison of mutant enzymes with native PPases revealed that a single amino acid substitution changes the position of the mutated residue as well as the positions of several functional groups and some parts of a polypeptide chain. These changes are responsible for the fact that mutant PPases differ from the native ones in their catalytic properties. The sulfate binding to the mutant PPase active site causes molecular asymmetry, as shown for the native PPase earlier. The subunit asymmetry is manifested in different positions of sulfate and several functional groups, as well as changes in packing of hexamers in crystals and in cell parameters.
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==About this Structure==
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1MJX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJX OCA].
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==Reference==
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Three-dimensional structures of mutant forms of E. coli inorganic pyrophosphatase with Asp--&gt;Asn single substitution in positions 42, 65, 70, and 97., Avaeva SM, Rodina EV, Vorobyeva NN, Kurilova SA, Nazarova TI, Sklyankina VA, Oganessyan VY, Samygina VR, Harutyunyan EH, Biochemistry (Mosc). 1998 Jun;63(6):671-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9668207 9668207]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Inorganic diphosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Avaeva SM]]
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[[Category: Avaeva, S M.]]
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[[Category: Harutyunyan EH]]
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[[Category: Harutyunyan, E H.]]
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[[Category: Huber R]]
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[[Category: Huber, R.]]
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[[Category: Oganesyan V]]
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[[Category: Oganesyan, V.]]
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[[Category: SO4]]
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[[Category: acid anhydride hydrolase]]
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[[Category: hydrolase]]
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[[Category: mutation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:44:19 2008''
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Current revision

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D65N

PDB ID 1mjx

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