1mme

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[[Image:1mme.jpg|left|200px]]<br /><applet load="1mme" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mme, resolution 3.100&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE==
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We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
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<StructureSection load='1mme' size='340' side='right'caption='[[1mme]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MME FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [https://pdbe.org/1mme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [https://www.ebi.ac.uk/pdbsum/1mme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mme ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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1MME is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA].
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7541315 7541315]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Self-splicing RNA]]
[[Category: Self-splicing RNA]]
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[[Category: Finch, J T.]]
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[[Category: Finch JT]]
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[[Category: Klug, A.]]
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[[Category: Klug A]]
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[[Category: Scott, W G.]]
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[[Category: Scott WG]]
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[[Category: catalytic rna]]
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[[Category: loop]]
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[[Category: rna hammerhead ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:41 2008''
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THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE

PDB ID 1mme

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