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| - | [[Image:1mme.jpg|left|200px]] | |
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| - | {{Structure
| + | ==THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE== |
| - | |PDB= 1mme |SIZE=350|CAPTION= <scene name='initialview01'>1mme</scene>, resolution 3.100Å
| + | <StructureSection load='1mme' size='340' side='right'caption='[[1mme]], [[Resolution|resolution]] 3.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MME FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [https://pdbe.org/1mme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [https://www.ebi.ac.uk/pdbsum/1mme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mme ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [http://www.ebi.ac.uk/pdbsum/1mme PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB]</span>
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| - | }}
| + | |
| | | | |
| - | '''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE'''
| + | ==See Also== |
| - | | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: RCSB PDB Molecule of the Month]] |
| - | ==About this Structure==
| + | |
| - | 1MME is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7541315 7541315]
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| - | [[Category: Protein complex]] | + | |
| | [[Category: Self-splicing RNA]] | | [[Category: Self-splicing RNA]] |
| - | [[Category: Finch, J T.]] | + | [[Category: Finch JT]] |
| - | [[Category: Klug, A.]] | + | [[Category: Klug A]] |
| - | [[Category: Scott, W G.]] | + | [[Category: Scott WG]] |
| - | [[Category: catalytic rna]]
| + | |
| - | [[Category: loop]]
| + | |
| - | [[Category: rna hammerhead ribozyme]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:18:03 2008''
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