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1mpg

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[[Image:1mpg.gif|left|200px]]
 
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==3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI==
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The line below this paragraph, containing "STRUCTURE_1mpg", creates the "Structure Box" on the page.
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<StructureSection load='1mpg' size='340' side='right'caption='[[1mpg]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mpg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_1mpg| PDB=1mpg | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpg OCA], [https://pdbe.org/1mpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpg RCSB], [https://www.ebi.ac.uk/pdbsum/1mpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/3MG2_ECOLI 3MG2_ECOLI] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpg ConSurf].
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<div style="clear:both"></div>
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'''3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI'''
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==See Also==
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Base-excision DNA repair proteins that target alkylation damage act on a variety of seemingly dissimilar adducts, yet fail to recognize other closely related lesions. The 1.8 A crystal structure of the monofunctional DNA glycosylase AlkA (E. coli 3-methyladenine-DNA glycosylase II) reveals a large hydrophobic cleft unusually rich in aromatic residues. An Asp residue projecting into this cleft is essential for catalysis, and it governs binding specificity for mechanism-based inhibitors. We propose that AlkA recognizes electron-deficient methylated bases through pi-donor/acceptor interactions involving the electron-rich aromatic cleft. Remarkably, AlkA is similar in fold and active site location to the bifunctional glycosylase/lyase endonuclease III, suggesting the two may employ fundamentally related mechanisms for base excision.
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==About this Structure==
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1MPG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPG OCA].
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==Reference==
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Structural basis for the excision repair of alkylation-damaged DNA., Labahn J, Scharer OD, Long A, Ezaz-Nikpay K, Verdine GL, Ellenberger TE, Cell. 1996 Jul 26;86(2):321-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8706136 8706136]
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[[Category: DNA-3-methyladenine glycosylase II]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ellenberger, T E.]]
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[[Category: Ellenberger TE]]
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[[Category: Ezaz-Nikpay, K.]]
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[[Category: Ezaz-Nikpay K]]
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[[Category: Labahn, J.]]
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[[Category: Labahn J]]
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[[Category: Long, A.]]
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[[Category: Long A]]
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[[Category: Schaerer, O D.]]
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[[Category: Schaerer OD]]
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[[Category: Verdine, G L.]]
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[[Category: Verdine GL]]
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[[Category: Alka]]
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[[Category: Base excision]]
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[[Category: Dna glycosylase]]
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[[Category: Dna repair]]
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[[Category: Hydrolase]]
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[[Category: Methylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:33:38 2008''
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Current revision

3-METHYLADENINE DNA GLYCOSYLASE II FROM ESCHERICHIA COLI

PDB ID 1mpg

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