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1mvq

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[[Image:1mvq.gif|left|200px]]<br /><applet load="1mvq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mvq, resolution 1.77&Aring;" />
 
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'''Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose'''<br />
 
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==Overview==
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==Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose==
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Carbohydrate-protein interactions play a key role in many biological processes. Cramoll is a lectin purified from Cratylia mollis seeds that is taxonomically related to concanavalin A (Con A). Although Cramoll and Con A have the same monosaccharide specificity, they have different glycoprotein binding profiles. We report the primary structure of Cramoll, determined by Edman degradation and mass spectrometry and its 1.77 A crystallographic structure and compare it with the three-dimensional structure of Con A in an attempt to understand how differential binding can be achieved by similar or nearly identical structures. We report here that Cramoll consists of 236 residues, with 82% identity with Con A, and that its topological architecture is essentially identical to Con A, because the Calpha positional differences are below 3.5 A. Cramoll and Con A have identical binding sites for MealphaMan, Mn2+, and Ca2+. However, we observed six substitutions in a groove adjacent to the extended binding site and two in the extended binding site that may explain the differences in binding of oligosaccharides and glycoproteins between Cramoll and Con A.
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<StructureSection load='1mvq' size='340' side='right'caption='[[1mvq]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cratylia_mollis Cratylia mollis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVQ FirstGlance]. <br>
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1MVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cratylia_mollis Cratylia mollis] with <scene name='pdbligand=MMA:'>MMA</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvq OCA], [https://pdbe.org/1mvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvq RCSB], [https://www.ebi.ac.uk/pdbsum/1mvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvq ProSAT]</span></td></tr>
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Amino acid sequence and tertiary structure of Cratylia mollis seed lectin., De Souza GA, Oliveira PS, Trapani S, Santos AC, Rosa JC, Laure HJ, Faca VM, Correia MT, Tavares GA, Oliva G, Coelho LC, Greene LJ, Glycobiology. 2003 Dec;13(12):961-72. Epub 2003 Sep 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12966038 12966038]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEC1_CRAMO LEC1_CRAMO] Glucose/D-mannose specific lectin.<ref>PMID:8579345</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvq ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cratylia mollis]]
[[Category: Cratylia mollis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Coelho, L C.]]
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[[Category: Coelho LC]]
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[[Category: Correia, M T.]]
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[[Category: Correia MT]]
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[[Category: Greene, L J.]]
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[[Category: Greene LJ]]
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[[Category: Oliva, G.]]
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[[Category: Oliva G]]
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[[Category: Oliveira, P S.]]
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[[Category: Oliveira PS]]
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[[Category: Souza, G A.de.]]
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[[Category: Trapani S]]
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[[Category: Trapani, S.]]
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[[Category: De Souza GA]]
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[[Category: CA]]
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[[Category: MMA]]
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[[Category: MN]]
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[[Category: legume lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:30 2008''
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Current revision

Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose

PDB ID 1mvq

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