This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1myk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:50, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1myk.gif|left|200px]]<br /><applet load="1myk" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1myk, resolution 2.4&Aring;" />
 
-
'''CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8==
-
Arc repressor is a small, dimeric DNA-binding protein that belongs to the ribbon-helix-helix family of transcription factors. Replacing Pro8 at the N-terminal end of the beta-sheet with leucine increases the stability of the mutant protein by 2.5 kcal/mol of dimer. However, this enhanced stability is achieved at the expense of significantly reduced DNA binding affinity. The structure of the PL8 mutant dimer has been determined to 2.4-A resolution by X-ray crystallography. The overall structure of the mutant is very similar to wild type, but Leu8 makes an additional interstrand hydrogen bond at each end of the beta-sheet of the mutant, increasing the total number of beta-sheet hydrogen bonds from six to eight. Comparison of the refolding and unfolding kinetics of the PL8 mutant and wild-type Arc shows that the enhanced stability of the mutant is accounted for by a decrease in the rate of protein unfolding, suggesting that the mutation acts to stabilize the native state and that the beta-sheet forms after the rate-limiting step in folding. The reduced operator affinity of the PL8 dimer appears to arise because the mutant cannot make the new interstrand hydrogen bonds and simultaneously make the wild-type set of contacts with operator DNA.
+
<StructureSection load='1myk' size='340' side='right'caption='[[1myk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1myk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MYK FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1myk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1myk OCA], [https://pdbe.org/1myk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1myk RCSB], [https://www.ebi.ac.uk/pdbsum/1myk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1myk ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
-
==About this Structure==
+
==See Also==
-
1MYK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_phage_py54 Yersinia phage py54]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA].
+
*[[Myosin 3D Structures|Myosin 3D Structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure, folding, and operator binding of the hyperstable Arc repressor mutant PL8., Schildbach JF, Milla ME, Jeffrey PD, Raumann BE, Sauer RT, Biochemistry. 1995 Jan 31;34(4):1405-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7827088 7827088]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Salmonella virus P22]]
-
[[Category: Yersinia phage py54]]
+
[[Category: Jeffrey PD]]
-
[[Category: Jeffrey, P D.]]
+
[[Category: Milla ME]]
-
[[Category: Milla, M E.]]
+
[[Category: Raumann BE]]
-
[[Category: Raumann, B E.]]
+
[[Category: Sauer RT]]
-
[[Category: Sauer, R T.]]
+
[[Category: Schildbach JF]]
-
[[Category: Schildbach, J F.]]
+
-
[[Category: transcription regulation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:23 2008''
+

Current revision

CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8

PDB ID 1myk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1myk"
Personal tools