1n4k

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(New page: 200px<br /><applet load="1n4k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n4k, resolution 2.20&Aring;" /> '''Crystal structure of...)
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[[Image:1n4k.jpg|left|200px]]<br /><applet load="1n4k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n4k, resolution 2.20&Aring;" />
 
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'''Crystal structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with IP3'''<br />
 
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==Overview==
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==Crystal structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with IP3==
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In a variety of cells, the Ca2+ signalling process is mediated by the, endoplasmic-reticulum-membrane-associated Ca2+ release channel, inositol, 1,4,5-trisphosphate (InsP3) receptor (InsP3R). Being ubiquitous and, present in organisms ranging from humans to Caenorhabditis elegans, InsP3R, has a vital role in the control of cellular and physiological processes as, diverse as cell division, cell proliferation, apoptosis, fertilization, development, behaviour, memory and learning. Mouse type I InsP3R, (InsP3R1), found in high abundance in cerebellar Purkinje cells, is a, polypeptide with three major functionally distinct regions: the, amino-terminal InsP3-binding region, the central modulatory region and the, carboxy-terminal channel region. Here we present a 2.2-A crystal structure, of the InsP3-binding core of mouse InsP3R1 in complex with InsP3. The, asymmetric, boomerang-like structure consists of an N-terminal, beta-trefoil domain and a C-terminal alpha-helical domain containing an, 'armadillo repeat'-like fold. The cleft formed by the two domains exposes, a cluster of arginine and lysine residues that coordinate the three, phosphoryl groups of InsP3. Putative Ca2+-binding sites are identified in, two separate locations within the InsP3-binding core.
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<StructureSection load='1n4k' size='340' side='right'caption='[[1n4k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n4k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I3P:D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE'>I3P</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4k OCA], [https://pdbe.org/1n4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4k RCSB], [https://www.ebi.ac.uk/pdbsum/1n4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ITPR1_MOUSE ITPR1_MOUSE] Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.<ref>PMID:2554142</ref> <ref>PMID:19752026</ref> <ref>PMID:20813840</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4k ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with I3P as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N4K OCA].
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*[[Inositol 1%2C4%2C5-Trisphosphate Receptor|Inositol 1%2C4%2C5-Trisphosphate Receptor]]
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== References ==
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==Reference==
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<references/>
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Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand., Bosanac I, Alattia JR, Mal TK, Chan J, Talarico S, Tong FK, Tong KI, Yoshikawa F, Furuichi T, Iwai M, Michikawa T, Mikoshiba K, Ikura M, Nature. 2002 Dec 12;420(6916):696-700. Epub 2002 Nov 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12442173 12442173]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Alattia JR]]
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[[Category: Alattia, J.R.]]
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[[Category: Bosanac I]]
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[[Category: Bosanac, I.]]
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[[Category: Chan J]]
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[[Category: Chan, J.]]
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[[Category: Furuichi T]]
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[[Category: Furuichi, T.]]
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[[Category: Ikura M]]
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[[Category: Ikura, M.]]
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[[Category: Iwai M]]
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[[Category: Iwai, M.]]
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[[Category: Mal TK]]
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[[Category: Mal, T.K.]]
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[[Category: Michikawa T]]
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[[Category: Michikawa, T.]]
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[[Category: Mikoshiba K]]
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[[Category: Mikoshiba, K.]]
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[[Category: Talarico S]]
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[[Category: Talarico, S.]]
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[[Category: Tong FK]]
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[[Category: Tong, F.K.]]
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[[Category: Tong KI]]
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[[Category: Tong, K.I.]]
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[[Category: Yoshikawa F]]
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[[Category: Yoshikawa, F.]]
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[[Category: I3P]]
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[[Category: armadillo-like fold]]
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[[Category: b-trefoil fold]]
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[[Category: calcium channel]]
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[[Category: ip3 receptor]]
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[[Category: ip3-binding core]]
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[[Category: protein-ligand complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:57:25 2007''
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Current revision

Crystal structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with IP3

PDB ID 1n4k

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