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1n7s

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(New page: 200px<br /><applet load="1n7s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7s, resolution 1.45&Aring;" /> '''High Resolution Stru...)
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[[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n7s, resolution 1.45&Aring;" />
 
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'''High Resolution Structure of a Truncated Neuronal SNARE Complex'''<br />
 
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==Overview==
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==High Resolution Structure of a Truncated Neuronal SNARE Complex==
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The structure of a truncated SNARE complex has been solved to 1.4-A, resolution revealing a stabilizing salt bridge, sites of hydration, and, conformational variability of the ionic central layer that were not, observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid, binding and denatures at a lower temperature than longer complexes as, assessed by SDS and circular dichroism thermal melts. The truncated SNARE, complex is monomeric, and it retains binding to synaptotagmin I.
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<StructureSection load='1n7s' size='340' side='right'caption='[[1n7s]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n7s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7s OCA], [https://pdbe.org/1n7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7s RCSB], [https://www.ebi.ac.uk/pdbsum/1n7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7s ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA].
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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*[[Vesicle-associated membrane protein|Vesicle-associated membrane protein]]
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==Reference==
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__TOC__
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High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex., Ernst JA, Brunger AT, J Biol Chem. 2003 Mar 7;278(10):8630-6. Epub 2002 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12496247 12496247]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Brunger, A.T.]]
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[[Category: Brunger AT]]
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[[Category: Ernst, J.A.]]
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[[Category: Ernst JA]]
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[[Category: CA]]
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[[Category: MPD]]
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[[Category: four helix bundle]]
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[[Category: neuronal snare protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:01:51 2007''
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Current revision

High Resolution Structure of a Truncated Neuronal SNARE Complex

PDB ID 1n7s

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