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1ncx

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(New page: 200px<br /><applet load="1ncx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ncx, resolution 1.8&Aring;" /> '''TROPONIN C'''<br /> ...)
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[[Image:1ncx.jpg|left|200px]]<br /><applet load="1ncx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ncx, resolution 1.8&Aring;" />
 
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'''TROPONIN C'''<br />
 
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==Overview==
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==TROPONIN C==
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The crystal structures of three metal complexes of troponin C (TnC) have, been determined and refined where the two occupied structural Ca(2+) sites, in the C domain have been substituted by Mn(2+), Cd(2+) and Tb(3+). The, X-ray intensity data were collected to 2.1, 1.8 and 1.8 A resolution, respectively, on the three metal complexes, which are isomorphous with, Ca-TnC. The three complexes have r.m.s. deviations of 0.27, 0.25 and 0.35, A, respectively, for all protein atoms, from Ca-TnC. Irrespective of the, charge on the metal (+2 or +3), the occupied sites 3 and 4 exhibit a, distorted pentagonal bipyramidal coordination, like Ca-TnC, with seven, ligands, six from the 12-residue binding loop and the seventh from a water, molecule. Mn(2+) at site 4 seems to display a longer distance to one of, the carboxyl bidentate ligands representing an intermediate coordination, simulating the six-coordinate Mg(2+). The carboxyl O atoms of the, bidentate Glu12 are displaced on the side of the equatorial plane passing, through the remaining three ligands with one O atom closer to the plane, (Delta of 0.11 to 0.76 A) than the other (Delta of 0.93 to 1.38 A). The, two axial ligands are an aspartic carboxyl O atom and a water molecule., The metal is displaced (0.18 to 0.56 A) towards the water facing the water, channel.
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<StructureSection load='1ncx' size='340' side='right'caption='[[1ncx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ncx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NCX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ncx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ncx OCA], [https://pdbe.org/1ncx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ncx RCSB], [https://www.ebi.ac.uk/pdbsum/1ncx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ncx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNNC2_CHICK TNNC2_CHICK] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nc/1ncx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ncx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NCX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CD and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NCX OCA].
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*[[Troponin 3D structures|Troponin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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X-ray structures of Mn, Cd and Tb metal complexes of troponin C., Rao ST, Satyshur KA, Greaser ML, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):916-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299599 15299599]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Rao, S.T.]]
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[[Category: Rao ST]]
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[[Category: Sundaralingam, M.]]
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[[Category: Sundaralingam M]]
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[[Category: CD]]
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[[Category: SO4]]
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[[Category: calcium-binding protein]]
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[[Category: duplication]]
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[[Category: muscle protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:08:52 2007''
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Current revision

TROPONIN C

PDB ID 1ncx

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