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1nfh

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[[Image:1nfh.gif|left|200px]]<br /><applet load="1nfh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nfh, resolution 2.65&Aring;" />
 
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'''Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding'''<br />
 
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==Overview==
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==Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding==
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The targeted acetylation status of histones and several other transcriptional regulatory proteins plays an important role in gene expression, although the mechanism for this is not well understood. As a model to understand how targeted acetylation may effect transcription, we determined the x-ray crystal structure of the chromatin protein Alba from Archaeoglobus fulgidus, a substrate for the Sir2 protein that deacetylates it at lysine 11 to promote DNA binding by Alba. The structure reveals a dimer of dimers in which the dimer-dimer interface is stabilized by several conserved hydrophobic residues as well as the lysine 11 target of Sir2. We show that, in solution, the mutation of these hydrophobic residues or lysine 11 disrupts dimer-dimer formation and decreases DNA-binding affinity. We propose that the in vivo deacetylation of lysine 11 of archaeal Alba by Sir2 promotes protein oligomerization for optimal DNA binding. Implications for the mechanism by which histone acetylation modulates gene expression are discussed.
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<StructureSection load='1nfh' size='340' side='right'caption='[[1nfh]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nfh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NFH FirstGlance]. <br>
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1NFH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nfh OCA], [https://pdbe.org/1nfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nfh RCSB], [https://www.ebi.ac.uk/pdbsum/1nfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nfh ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of a Sir2 substrate, Alba, reveals a mechanism for deacetylation-induced enhancement of DNA binding., Zhao K, Chai X, Marmorstein R, J Biol Chem. 2003 Jul 11;278(28):26071-7. Epub 2003 May 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12730210 12730210]
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== Function ==
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[https://www.uniprot.org/uniprot/ALBA2_ARCFU ALBA2_ARCFU] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).[HAMAP-Rule:MF_01122]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nfh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nfh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chai, X.]]
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[[Category: Chai X]]
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[[Category: Marmorstein, R.]]
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[[Category: Marmorstein R]]
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[[Category: Zhao, K.]]
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[[Category: Zhao K]]
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[[Category: alba]]
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[[Category: gene regulation]]
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[[Category: hdac]]
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[[Category: sir2]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:29 2008''
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Current revision

Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding

PDB ID 1nfh

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