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1nlc

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[[Image:1nlc.jpg|left|200px]]<br /><applet load="1nlc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nlc, resolution 1.85&Aring;" />
 
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'''HIV-1 DIS(Mal) duplex Zn-soaked'''<br />
 
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==Overview==
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==HIV-1 DIS(Mal) duplex Zn-soaked==
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Metal ions, and magnesium in particular, are known to be involved in RNA, folding by stabilizing secondary and tertiary structures, and, as, cofactors, in RNA enzymatic activity. We have conducted a systematic, crystallographic analysis of cation binding to the duplex form of the, HIV-1 RNA dimerization initiation site for the subtype-A and -B natural, sequences. Eleven ions (K+, Pb2+, Mn2+, Ba2+, Ca2+, Cd2+, Sr2+, Zn2+, Co2+, Au3+ and Pt4+) and two hexammines [Co (NH3)6]3+ and [Ru (NH3)6]3+, were found to bind to the DIS duplex structure. Although the two sequences, are very similar, strong differences were found in their cation binding, properties. Divalent cations bind almost exclusively, as Mg2+, at, 'Hoogsteen' sites of guanine residues, with a cation-dependent affinity, for each site. Notably, a given cation can have very different affinities, for a priori equivalent sites within the same molecule. Surprisingly, none, of the two hexammines used were able to efficiently replace hexahydrated, magnesium. Instead, [Co (NH3)4]3+ was seen bound by inner-sphere, coordination to the RNA. This raises some questions about the practical, use of [Co (NH3)6]3+ as a [Mg (H2O)6]2+ mimetic. Also very unexpected was, the binding of the small Au3+ cation exactly between the Watson-Crick, sites of a G-C base pair after an obligatory deprotonation of N1 of the, guanine base. This extensive study of metal ion binding using X-ray, crystallography significantly enriches our knowledge on the binding of, middleweight or heavy metal ions to RNA, particularly compared with, magnesium.
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<StructureSection load='1nlc' size='340' side='right'caption='[[1nlc]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nlc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NLC FirstGlance]. <br>
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1NLC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLC OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlc OCA], [https://pdbe.org/1nlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nlc RCSB], [https://www.ebi.ac.uk/pdbsum/1nlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nlc ProSAT]</span></td></tr>
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A crystallographic study of the binding of 13 metal ions to two related RNA duplexes., Ennifar E, Walter P, Dumas P, Nucleic Acids Res. 2003 May 15;31(10):2671-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12736317 12736317]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Dumas, P.]]
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</StructureSection>
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[[Category: Ennifar, E.]]
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[[Category: Large Structures]]
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[[Category: Walter, P.]]
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[[Category: Dumas P]]
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[[Category: ZN]]
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[[Category: Ennifar E]]
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[[Category: hiv-1]]
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[[Category: Walter P]]
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[[Category: rna]]
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[[Category: zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:29:24 2008''
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Current revision

HIV-1 DIS(Mal) duplex Zn-soaked

PDB ID 1nlc

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