1no4

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(New page: 200px<br /><applet load="1no4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1no4, resolution 2.20&Aring;" /> '''Crystal Structure of...)
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[[Image:1no4.gif|left|200px]]<br /><applet load="1no4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1no4, resolution 2.20&Aring;" />
 
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'''Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29'''<br />
 
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==Overview==
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==Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29==
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Three-dimensional structures of the double-stranded DNA bacteriophage, phi29 scaffolding protein (gp7) before and after prohead assembly have, been determined at resolutions of 2.2 and 2.8 A, respectively. Both, structures are dimers that resemble arrows, with a four-helix bundle, composing the arrowhead and a coiled coil forming the tail. The structural, resemblance of gp7 to the yeast transcription factor GCN4 suggests a, DNA-binding function that was confirmed by native gel electrophoresis. DNA, binding to gp7 may have a role in mediating the structural transition from, prohead to mature virus and scaffold release. A cryo-EM analysis indicates, that gp7 is arranged inside the capsid as a series of concentric shells., The position of the higher density features in these shells correlates, with the positions of hexamers in the equatorial region of the capsid, suggesting that gp7 may regulate formation of the prolate head through, interactions with these hexamers.
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<StructureSection load='1no4' size='340' side='right'caption='[[1no4]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1no4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NO4 FirstGlance]. <br>
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1NO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NO4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1no4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1no4 OCA], [https://pdbe.org/1no4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1no4 RCSB], [https://www.ebi.ac.uk/pdbsum/1no4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1no4 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12778115 12778115]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/SCAF_BPPH2 SCAF_BPPH2] Scaffolding protein involved in the icosahedric procapsid assembly. Coassembles with the capsid proteins to form the procapsid, in which the scaffolding protein is found within the external shell of icosahedrally arranged capsid protein subunits. In a subsequent step the scaffolding protein molecules are released from the procapsid.<ref>PMID:17098197</ref> <ref>PMID:17198713</ref> <ref>PMID:23896641</ref>
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[[Category: Vibrio phage f237]]
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== References ==
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[[Category: Anderson, D.L.]]
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<references/>
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[[Category: Badasso, M.O.]]
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__TOC__
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[[Category: Kanamaru, S.]]
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</StructureSection>
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[[Category: Koti, J.S.]]
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[[Category: Bacillus virus phi29]]
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[[Category: McMurray, C.T.]]
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[[Category: Large Structures]]
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[[Category: Morais, M.C.]]
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[[Category: Anderson DL]]
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[[Category: Owen, B.A.L.]]
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[[Category: Badasso MO]]
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[[Category: Rossmann, M.G.]]
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[[Category: Kanamaru S]]
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[[Category: coiled-coil]]
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[[Category: Koti JS]]
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[[Category: McMurray CT]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:25:15 2007''
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[[Category: Morais MC]]
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[[Category: Owen BAL]]
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[[Category: Rossmann MG]]

Current revision

Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29

PDB ID 1no4

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