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1np8

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[[Image:1np8.jpg|left|200px]]<br /><applet load="1np8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1np8, resolution 2.00&Aring;" />
 
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'''18-k C-terminally trunucated small subunit of calpain'''<br />
 
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==Overview==
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==18-k C-terminally trunucated small subunit of calpain==
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The subunits in calpain and in the related penta-EF-hand (PEF) proteins are bound through contacts between the unpaired EF-hand 5 from each subunit. To study subunit binding further, a tetra-EF-hand 18 kDa N- and C-terminally truncated form of the calpain small subunit was prepared (18k). This protein does not combine with the calpain large subunit to form active calpain, but forms homodimers in solution, as shown by ultracentrifugation. The X-ray structure of the 18k protein in the presence of cadmium was solved to a resolution of 2.0 A. The structure of the monomer is almost identical to the known structure of the calpain small subunit, but the 18k protein forms an oligomer in the crystal by the use of two binding sites. One of these sites is an artefact arising from the C-terminal truncation, but the other is a naturally occurring site that is fully exposed to water in intact purified calpain. The characteristics of this site suggest that it may be important in binding other protein modulators involved in the regulation of calpain and of PEF proteins.
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<StructureSection load='1np8' size='340' side='right'caption='[[1np8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1np8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NP8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1np8 OCA], [https://pdbe.org/1np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1np8 RCSB], [https://www.ebi.ac.uk/pdbsum/1np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1np8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPNS1_RAT CPNS1_RAT] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1np8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1np8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NP8 OCA].
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*[[Calpain 3D structures|Calpain 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A second binding site revealed by C-terminal truncation of calpain small subunit, a penta-EF-hand protein., Leinala EK, Arthur JS, Grochulski P, Davies PL, Elce JS, Jia Z, Proteins. 2003 Nov 15;53(3):649-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14579356 14579356]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Arthur JS]]
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[[Category: Arthur, J S.]]
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[[Category: Davies PL]]
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[[Category: Davies, P L.]]
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[[Category: Elce JS]]
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[[Category: Elce, J S.]]
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[[Category: Grochulski P]]
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[[Category: Grochulski, P.]]
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[[Category: Jia Z]]
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[[Category: Jia, Z.]]
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[[Category: Leinala EK]]
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[[Category: Leinala, E K.]]
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[[Category: CD]]
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[[Category: dimer in solution]]
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[[Category: oligomer in crystal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:32 2008''
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Current revision

18-k C-terminally trunucated small subunit of calpain

PDB ID 1np8

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