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1nqt

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[[Image:1nqt.gif|left|200px]]
 
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{{Structure
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==Crystal structure of bovine Glutamate dehydrogenase-ADP complex==
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|PDB= 1nqt |SIZE=350|CAPTION= <scene name='initialview01'>1nqt</scene>, resolution 3.50&Aring;
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<StructureSection load='1nqt' size='340' side='right'caption='[[1nqt]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[1nqt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NQT FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glutamate_dehydrogenase_(NAD(P)(+)) Glutamate dehydrogenase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.3 1.4.1.3]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqt OCA], [https://pdbe.org/1nqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nqt RCSB], [https://www.ebi.ac.uk/pdbsum/1nqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHE3_BOVIN DHE3_BOVIN] May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).<ref>PMID:14659072</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/1nqt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nqt ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of bovine Glutamate dehydrogenase-ADP complex'''
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==See Also==
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*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Glutamate dehydrogenase (GDH) is found in all organisms and catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate. Unlike GDH from bacteria, mammalian GDH exhibits negative cooperativity with respect to coenzyme, activation by ADP, and inhibition by GTP. Presented here are the structures of apo bovine GDH, bovine GDH complexed with ADP, and the R463A mutant form of human GDH (huGDH) that is insensitive to ADP activation. In the absence of active site ligands, the catalytic cleft is in the open conformation, and the hexamers form long polymers in the crystal cell with more interactions than found in the abortive complex crystals. This is consistent with the fact that ADP promotes aggregation in solution. ADP is shown to bind to the second, inhibitory, NADH site yet causes activation. The beta-phosphates of the bound ADP interact with R459 (R463 in huGDH) on the pivot helix. The structure of the ADP-resistant, R463A mutant of human GDH is identical to native GDH with the exception of the truncated side chain on the pivot helix. Together, these results strongly suggest that ADP activates by facilitating the opening of the catalytic cleft. From alignment of GDH from various sources, it is likely that the antenna evolved in the protista prior to the formation of purine regulatory sites. This suggests that there was some selective advantage of the antenna itself and that animals evolved new functions for GDH through the addition of allosteric regulation.
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__TOC__
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</StructureSection>
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==About this Structure==
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1NQT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQT OCA].
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==Reference==
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Structural studies on ADP activation of mammalian glutamate dehydrogenase and the evolution of regulation., Banerjee S, Schmidt T, Fang J, Stanley CA, Smith TJ, Biochemistry. 2003 Apr 1;42(12):3446-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12653548 12653548]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Glutamate dehydrogenase (NAD(P)(+))]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Banerjee S]]
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[[Category: Banerjee, S.]]
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[[Category: Fang J]]
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[[Category: Fang, J.]]
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[[Category: Schmidt T]]
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[[Category: Schmidt, T.]]
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[[Category: Smith TJ]]
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[[Category: Smith, T J.]]
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[[Category: Stanley CA]]
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[[Category: Stanley, C A.]]
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[[Category: ADP]]
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[[Category: dimer of two hexamer]]
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[[Category: glutamate dehydrogenase-adp complex]]
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[[Category: regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:00:29 2008''
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Current revision

Crystal structure of bovine Glutamate dehydrogenase-ADP complex

PDB ID 1nqt

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