1on2

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[[Image:1on2.gif|left|200px]]
 
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==Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese==
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The line below this paragraph, containing "STRUCTURE_1on2", creates the "Structure Box" on the page.
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<StructureSection load='1on2' size='340' side='right'caption='[[1on2]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1on2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ON2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ON2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_1on2| PDB=1on2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1on2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1on2 OCA], [https://pdbe.org/1on2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1on2 RCSB], [https://www.ebi.ac.uk/pdbsum/1on2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1on2 ProSAT]</span></td></tr>
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</table>
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'''Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese'''
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== Function ==
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[https://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The Bacillus subtilis manganese transport regulator, MntR, binds Mn2+ as an effector and is a repressor of transporters that import manganese. A member of the diphtheria toxin repressor (DtxR) family of metalloregulatory proteins, MntR exhibits selectivity for Mn2+ over Fe2+. Replacement of a metal-binding residue, Asp8, with methionine (D8M) relaxes this specificity. We report here the X-ray crystal structures of wild-type MntR and the D8M mutant bound to manganese with 1.75 A and 1.61 A resolution, respectively. The 142-residue MntR homodimer has substantial structural similarity to the 226-residue DtxR but lacks the C-terminal SH3-like domain of DtxR. The metal-binding pockets of MntR and DtxR are substantially different. The cation-to-cation distance between the two manganese ions bound by MntR is 3.3 A, whereas that between the metal ions bound by DtxR is 9 A. D8M binds only a single Mn2+ per monomer, owing to alteration of the metal-binding site. The sole retained metal site adopts pseudo-hexacoordinate geometry rather than the pseudo-heptacoordinate geometry of the MntR metal sites.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/1on2_consurf.spt"</scriptWhenChecked>
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1ON2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ON2 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the manganese-bound manganese transport regulator of Bacillus subtilis., Glasfeld A, Guedon E, Helmann JD, Brennan RG, Nat Struct Biol. 2003 Aug;10(8):652-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12847518 12847518]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1on2 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brennan, R G.]]
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[[Category: Brennan RG]]
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[[Category: Glasfeld, A.]]
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[[Category: Glasfeld A]]
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[[Category: Guedon, E.]]
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[[Category: Guedon E]]
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[[Category: Helmann, J D.]]
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[[Category: Helmann JD]]
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[[Category: Dna-binding protein]]
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[[Category: Helix-turn-helix]]
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[[Category: Metalloregulatory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:02:58 2008''
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Current revision

Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese

PDB ID 1on2

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