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1opy

From Proteopedia

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[[Image:1opy.gif|left|200px]]<br />
 
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<applet load="1opy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1opy, resolution 1.9&Aring;" />
 
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'''KSI'''<br />
 
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==Overview==
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==KSI==
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The three-dimensional structure of the enzyme 3-oxo-delta5-steroid, isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by, multidimensional heteronuclear magnetic resonance spectroscopy. The two, independently folded monomers pack together by means of extensive, hydrophobic and electrostatic interactions. Each monomer comprises three, alpha helices and a six-strand mixed beta-pleated sheet arranged to form a, deep hydrophobic cavity. Catalytically important residues Tyr14 (general, acid) and Asp38 (general base) are located near the bottom of the cavity, and positioned as expected from mechanistic hypotheses. An unexpected acid, group (Asp99) is also located in the active site adjacent to Tyr14, and, kinetic and binding studies of the Asp99 to Ala mutant demonstrate that, Asp99 contributes to catalysis by stabilizing the intermediate.
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<StructureSection load='1opy' size='340' side='right'caption='[[1opy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1opy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OPY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1opy OCA], [https://pdbe.org/1opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1opy RCSB], [https://www.ebi.ac.uk/pdbsum/1opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1opy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SDIS_PSEPU SDIS_PSEPU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1opy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1opy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Structure known Active Site: A. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA].
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*[[Ketosteroid Isomerase|Ketosteroid Isomerase]]
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__TOC__
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==Reference==
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</StructureSection>
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Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9103200 9103200]
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[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Cha S-S]]
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[[Category: Steroid Delta-isomerase]]
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[[Category: Cho H-S]]
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[[Category: Cha, S.S.]]
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[[Category: Cho M-J]]
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[[Category: Cho, H.S.]]
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[[Category: Choi K-Y]]
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[[Category: Cho, M.J.]]
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[[Category: Ha N-C]]
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[[Category: Choi, K.Y.]]
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[[Category: Kim J-S]]
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[[Category: Ha, N.C.]]
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[[Category: Kim S-W]]
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[[Category: Kim, J.S.]]
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[[Category: Oh B-H]]
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[[Category: Kim, S.W.]]
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[[Category: Oh, B.H.]]
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[[Category: high resolution]]
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[[Category: isomerase]]
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[[Category: ksi]]
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[[Category: low-barrier hydrogen bond]]
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[[Category: pseudomonas putida biotype b]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:58:41 2007''
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Current revision

KSI

PDB ID 1opy

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