1os7

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[[Image:1os7.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5==
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|PDB= 1os7 |SIZE=350|CAPTION= <scene name='initialview01'>1os7</scene>, resolution 2.5&Aring;
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<StructureSection load='1os7' size='340' side='right'caption='[[1os7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AKG:2-OXYGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene>
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<table><tr><td colspan='2'>[[1os7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OS7 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Taurine_dioxygenase Taurine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.17 1.14.11.17] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1os7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1os7 OCA], [https://pdbe.org/1os7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1os7 RCSB], [https://www.ebi.ac.uk/pdbsum/1os7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1os7 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1gqw|1GQW]], [[1gy9|1GY9]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1os7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1os7 OCA], [http://www.ebi.ac.uk/pdbsum/1os7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1os7 RCSB]</span>
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== Function ==
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[https://www.uniprot.org/uniprot/TAUD_ECOLI TAUD_ECOLI] Catalyzes the conversion of taurine and alpha ketoglutarate to sulfite, aminoacetaldehyde and succinate. Required for the utilization of taurine (2-aminoethanesulfonic acid) as an alternative sulfur source. Pentane-sulfonic acid, 3-(N-morpholino)propanesulfonic acid and 1,3-dioxo-2-isoindolineethanesulfonic acid are also substrates for this enzyme.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1os7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1os7 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5'''
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==See Also==
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*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonate (taurine) to sulfite and aminoacetaldehyde while decomposing alphaKG to succinate and CO(2). This alphaKG dependent dioxygenase is expressed in Escherichia coli under sulfur starvation conditions and allows the cell to utilize taurine, and other similar sulfonates in the environment, as an alternative sulfur source. In this work, we report the structures of the apo and holo forms of TauD to 1.9 A resolution (R(cryst) = 21.2%, R(free) = 24.9%) and 2.5 A resolution (R(cryst) = 22.5%, R(free) = 27.8%), respectively. The models reported herein provide significant new insight into the substrate orientations at the active site and the conformational changes that are induced upon taurine binding. Furthermore, analysis of our crystallographic data coupled with reanalysis of the crystallographic model (resolution = 3.0 A, R(cryst) = 28.1, R(free) = 32.0) presented by Elkins et al. (Biochemistry (2002) 41, 5185-5192) reveals an alternative oligomeric arrangement for the enzyme that is consistent with the conserved primary and secondary structure elements of other alphaKG dependent dioxygenases.
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==About this Structure==
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1OS7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OS7 OCA].
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==Reference==
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Substrate-induced conformational changes in Escherichia coli taurine/alpha-ketoglutarate dioxygenase and insight into the oligomeric structure., O'Brien JR, Schuller DJ, Yang VS, Dillard BD, Lanzilotta WN, Biochemistry. 2003 May 20;42(19):5547-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12741810 12741810]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Taurine dioxygenase]]
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[[Category: Dillard BD]]
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[[Category: Brien, J R.O.]]
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[[Category: Lanzilotta WN]]
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[[Category: Dillard, B D.]]
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[[Category: O'Brien JR]]
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[[Category: Lanzilotta, W N.]]
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[[Category: Schuller DJ]]
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[[Category: Schuller, D J.]]
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[[Category: Yang VS]]
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[[Category: Yang, V S.]]
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[[Category: alpha-ketoglutarate]]
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[[Category: iron di-oxygenase]]
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[[Category: taud]]
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[[Category: taurine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:38 2008''
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Current revision

Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5

PDB ID 1os7

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