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1oxz

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[[Image:1oxz.jpg|left|200px]]
 
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==Crystal Structure of the Human GGA1 GAT domain==
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The line below this paragraph, containing "STRUCTURE_1oxz", creates the "Structure Box" on the page.
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<StructureSection load='1oxz' size='340' side='right'caption='[[1oxz]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1oxz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxz OCA], [https://pdbe.org/1oxz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxz RCSB], [https://www.ebi.ac.uk/pdbsum/1oxz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxz ProSAT]</span></td></tr>
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{{STRUCTURE_1oxz| PDB=1oxz | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of the Human GGA1 GAT domain'''
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[https://www.uniprot.org/uniprot/GGA1_HUMAN GGA1_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other proteins in carrying out intracellular transport. The GAT domain of GGAs has been identified as the structural entity that binds membrane-bound ARF, a molecular switch regulating vesicle-coat assembly. It also directly interacts with rabaptin5, an essential component of endosome fusion. A 2.8 A resolution crystal structure of the human GGA1 GAT domain is reported here. The GAT domain contains four helices and has an elongated shape with the longest dimension exceeding 80 A. Its longest helix is involved in two structural motifs: an N-terminal helix-loop-helix motif and a C-terminal three-helix bundle. The N-terminal motif harbors the most conservative amino acid sequence in the GGA GAT domains. Within this conserved region, a cluster of residues previously implicated in ARF binding forms a hydrophobic surface patch, which is likely to be the ARF-binding site. In addition, a structure-based mutagenesis-biochemical analysis demonstrates that the C-terminal three-helix bundle of this GAT domain is responsible for the rabaptin5 binding. These structural characteristics are consistent with a model supporting multiple functional roles for the GAT domain.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxz_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1OXZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXZ OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oxz ConSurf].
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Crystal structure of the human GGA1 GAT domain., Zhu G, Zhai P, He X, Terzyan S, Zhang R, Joachimiak A, Tang J, Zhang XC, Biochemistry. 2003 Jun 3;42(21):6392-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12767220 12767220]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: He, X.]]
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[[Category: He X]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Tang, J.]]
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[[Category: Tang J]]
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[[Category: Terzyan, S.]]
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[[Category: Terzyan S]]
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[[Category: Zhai, P.]]
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[[Category: Zhai P]]
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[[Category: Zhang, R.]]
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[[Category: Zhang R]]
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[[Category: Zhang, X C.]]
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[[Category: Zhang XC]]
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[[Category: Zhu, G.]]
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[[Category: Zhu G]]
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[[Category: Gat domain]]
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[[Category: Gga1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:25:03 2008''
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Current revision

Crystal Structure of the Human GGA1 GAT domain

PDB ID 1oxz

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