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1oye

From Proteopedia

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[[Image:1oye.gif|left|200px]]
 
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{{Structure
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==Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump==
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|PDB= 1oye |SIZE=350|CAPTION= <scene name='initialview01'>1oye</scene>, resolution 3.48&Aring;
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<StructureSection load='1oye' size='340' side='right'caption='[[1oye]], [[Resolution|resolution]] 3.48&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CPF:1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC+ACID'>CPF</scene>
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<table><tr><td colspan='2'>[[1oye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OYE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.48&#8491;</td></tr>
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|GENE= ACRB OR ACRE OR B0462 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPF:1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC+ACID'>CPF</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oye OCA], [https://pdbe.org/1oye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oye RCSB], [https://www.ebi.ac.uk/pdbsum/1oye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oye ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oye OCA], [http://www.ebi.ac.uk/pdbsum/1oye PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oye RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
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== Evolutionary Conservation ==
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'''Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oy/1oye_consurf.spt"</scriptWhenChecked>
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Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1OYE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYE OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oye ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump., Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr, Science. 2003 May 9;300(5621):976-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12738864 12738864]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jr., D E.Koshland.]]
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[[Category: Koshland Jr DE]]
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[[Category: McDermott, G.]]
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[[Category: McDermott G]]
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[[Category: Nikaido, H.]]
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[[Category: Nikaido H]]
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[[Category: Yu, E W.]]
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[[Category: Yu EW]]
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[[Category: Zgurskaya, H I.]]
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[[Category: Zgurskaya HI]]
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[[Category: membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:08 2008''
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Current revision

Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump

PDB ID 1oye

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