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1p1i

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[[Image:1p1i.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the NAD+-bound 1L-myo-inositol 1-phosphate synthase==
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|PDB= 1p1i |SIZE=350|CAPTION= <scene name='initialview01'>1p1i</scene>, resolution 2.4&Aring;
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<StructureSection load='1p1i' size='340' side='right'caption='[[1p1i]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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<table><tr><td colspan='2'>[[1p1i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P1I FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p1i OCA], [https://pdbe.org/1p1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p1i RCSB], [https://www.ebi.ac.uk/pdbsum/1p1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p1i ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1p1f|1P1F]], [[1p1h|1P1H]], [[1p1j|1P1J]], [[1p1k|1P1K]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p1i OCA], [http://www.ebi.ac.uk/pdbsum/1p1i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p1i RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/INO1_YEAST INO1_YEAST]
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== Evolutionary Conservation ==
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'''Crystal structure of the NAD+-bound 1L-myo-inositol 1-phosphate synthase'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/1p1i_consurf.spt"</scriptWhenChecked>
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1-l-myo-Inositol 1-phosphate synthase catalyzes the conversion of d-glucose 6-phosphate to 1-l-myo-inositol 1-phosphate, the first and rate-limiting step in the biosynthesis of all inositol-containing compounds. It involves an oxidation, an intramolecular aldol cyclization and a reduction. Here, the structure of the enzyme in its NAD(+)-bound, NADH-bound and apo forms is presented. These structures confirm that a significant portion of the active site is disordered in the absence of a small molecule, as none of the NAD(+)-bound forms of the enzyme have ordered active sites. On the other hand, the NADH-bound form contains two small molecules in the active site: a phosphate and glycerol. The entire active site is ordered in the presence of these two molecules, completely encapsulating them within the interior cavity. Significant changes in the structure of the active site are also seen, including repositioning of the nicotinamide ring and a motion of a loop region to accommodate the bound phosphate. These changes call into question the mechanism previously proposed for the enzyme. A comparison of the yeast and mycobacterial enzymes shows a surprisingly large change in the relative orientation of the catalytic and Rossmann-fold domains in the two enzymes.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1P1I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1I OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p1i ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structures of NAD(+)- and NADH-bound 1-l-myo-inositol 1-phosphate synthase., Jin X, Geiger JH, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1154-64. Epub 2003, Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12832758 12832758]
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</StructureSection>
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[[Category: Inositol-3-phosphate synthase]]
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Geiger JH]]
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[[Category: Geiger, J H.]]
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[[Category: Jin X]]
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[[Category: Jin, X.]]
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[[Category: 1l-myo-inositol 1-phosphate]]
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[[Category: isomerase]]
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[[Category: nad+]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:53:31 2008''
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Current revision

Crystal structure of the NAD+-bound 1L-myo-inositol 1-phosphate synthase

PDB ID 1p1i

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