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1p1k

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(New page: 200px<br /><applet load="1p1k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p1k, resolution 2.1&Aring;" /> '''Crystal structure of ...)
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[[Image:1p1k.jpg|left|200px]]<br /><applet load="1p1k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p1k, resolution 2.1&Aring;" />
 
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'''Crystal structure of the 1L-myo-inositol 1-phosphate synthase complexed with NADH in the presence of EDTA'''<br />
 
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==Overview==
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==Crystal structure of the 1L-myo-inositol 1-phosphate synthase complexed with NADH in the presence of EDTA==
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1-l-myo-Inositol 1-phosphate synthase catalyzes the conversion of, d-glucose 6-phosphate to 1-l-myo-inositol 1-phosphate, the first and, rate-limiting step in the biosynthesis of all inositol-containing, compounds. It involves an oxidation, an intramolecular aldol cyclization, and a reduction. Here, the structure of the enzyme in its NAD(+)-bound, NADH-bound and apo forms is presented. These structures confirm that a, significant portion of the active site is disordered in the absence of a, small molecule, as none of the NAD(+)-bound forms of the enzyme have, ordered active sites. On the other hand, the NADH-bound form contains two, small molecules in the active site: a phosphate and glycerol. The entire, active site is ordered in the presence of these two molecules, completely, encapsulating them within the interior cavity. Significant changes in the, structure of the active site are also seen, including repositioning of the, nicotinamide ring and a motion of a loop region to accommodate the bound, phosphate. These changes call into question the mechanism previously, proposed for the enzyme. A comparison of the yeast and mycobacterial, enzymes shows a surprisingly large change in the relative orientation of, the catalytic and Rossmann-fold domains in the two enzymes.
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<StructureSection load='1p1k' size='340' side='right'caption='[[1p1k]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1p1k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P1K FirstGlance]. <br>
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1P1K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P1K OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p1k OCA], [https://pdbe.org/1p1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p1k RCSB], [https://www.ebi.ac.uk/pdbsum/1p1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p1k ProSAT]</span></td></tr>
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Structures of NAD(+)- and NADH-bound 1-l-myo-inositol 1-phosphate synthase., Jin X, Geiger JH, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1154-64. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832758 12832758]
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</table>
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[[Category: Inositol-3-phosphate synthase]]
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== Function ==
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[https://www.uniprot.org/uniprot/INO1_YEAST INO1_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/1p1k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p1k ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Geiger JH]]
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[[Category: Geiger, J.H.]]
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[[Category: Jin X]]
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[[Category: Jin, X.]]
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[[Category: NAI]]
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[[Category: 1l-myo-inositol 1-phosphate]]
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[[Category: edta]]
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[[Category: isomerase]]
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[[Category: nadh]]
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[[Category: rossmann fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:23:12 2007''
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Current revision

Crystal structure of the 1L-myo-inositol 1-phosphate synthase complexed with NADH in the presence of EDTA

PDB ID 1p1k

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