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| - | [[Image:1p99.gif|left|200px]] | |
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| - | {{Structure
| + | ==1.7A crystal structure of protein PG110 from Staphylococcus aureus== |
| - | |PDB= 1p99 |SIZE=350|CAPTION= <scene name='initialview01'>1p99</scene>, resolution 1.70Å
| + | <StructureSection load='1p99' size='340' side='right'caption='[[1p99]], [[Resolution|resolution]] 1.70Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene> | + | <table><tr><td colspan='2'>[[1p99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P99 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | |GENE= SA0422 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr> |
| - | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1464 NlpA]</span>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p99 OCA], [https://pdbe.org/1p99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p99 RCSB], [https://www.ebi.ac.uk/pdbsum/1p99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p99 ProSAT], [https://www.topsan.org/Proteins/MCSG/1p99 TOPSAN]</span></td></tr> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p99 OCA], [http://www.ebi.ac.uk/pdbsum/1p99 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1p99 RCSB]</span>
| + | </table> |
| - | }}
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/A0A0H3JTG5_STAAM A0A0H3JTG5_STAAM] |
| - | '''1.7A crystal structure of protein PG110 from Staphylococcus aureus'''
| + | == Evolutionary Conservation == |
| - | | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | ==Overview== | + | <jmolCheckbox> |
| - | Bacterial dipeptide ABC transporters function to import a wide range of dipeptide substrates. This ability to transport a wide variety of dipeptides is conferred by the cognate substrate binding protein (SBP) of these transporters. SBPs bind dipeptides with little regard for their amino acid content. Here, we report the 1.7 A resolution structure of lipoprotein-9 (SA0422) of Staphylococcus aureus in complex with the dipeptide glycylmethionine. Experimental characterization of the subcellular location of the protein confirmed that SA0422 is an acylated, peripheral membrane protein. This is the first structure determined for an SBP of a Gram-positive dipeptide ABC transporter. Usually, binding of dipeptides occurs in a binding pocket that is largely hydrated and able to accommodate the side chains of several different amino acid residues. Unlike any other known SBP, lipoprotein-9 binds the side chains of the glycylmethionine dipeptide through very specific interactions. Lipoprotein-9 shares significant structural and sequence homology with the MetQ family of methionine SBP. Sequence comparisons between MetQ-like proteins and lipoprotein-9 suggest that the residues forming the tight interactions with the methionine side chains of the ligand are highly conserved between lipoprotein-9 and MetQ homologues, while the residues involved in coordinating the glycine residue are not. Modeling of the Vibrio cholerae MetQ and lipoprotein-9 binding pockets can account for lipoprotein-9 substrate specificity toward glycylmethionine. For this reason, we have designated lipoprotein-9 GmpC, for glycylmethionine binding protein.
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p99_consurf.spt"</scriptWhenChecked> |
| - | | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | ==About this Structure== | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | 1P99 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P99 OCA].
| + | </jmolCheckbox> |
| - | | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p99 ConSurf]. |
| - | ==Reference== | + | <div style="clear:both"></div> |
| - | The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions., Williams WA, Zhang RG, Zhou M, Joachimiak G, Gornicki P, Missiakas D, Joachimiak A, Biochemistry. 2004 Dec 28;43(51):16193-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15610013 15610013]
| + | __TOC__ |
| - | [[Category: Single protein]] | + | </StructureSection> |
| - | [[Category: Staphylococcus aureus subsp. aureus mu50]] | + | [[Category: Large Structures]] |
| - | [[Category: Joachimiak, A.]] | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| - | [[Category: Joachimiak, G.]] | + | [[Category: Joachimiak A]] |
| - | [[Category: MCSG, Midwest Center for Structural Genomics.]]
| + | [[Category: Joachimiak G]] |
| - | [[Category: Schneewind, O.]] | + | [[Category: Schneewind O]] |
| - | [[Category: Zhang, R.]] | + | [[Category: Zhang R]] |
| - | [[Category: Zhou, M.]] | + | [[Category: Zhou M]] |
| - | [[Category: mcsg]]
| + | |
| - | [[Category: midwest center for structural genomic]]
| + | |
| - | [[Category: protein structure initiative]]
| + | |
| - | [[Category: psi]]
| + | |
| - | [[Category: structural genomic]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:00:24 2008''
| + | |
