1p99

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[[Image:1p99.gif|left|200px]]
 
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==1.7A crystal structure of protein PG110 from Staphylococcus aureus==
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The line below this paragraph, containing "STRUCTURE_1p99", creates the "Structure Box" on the page.
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<StructureSection load='1p99' size='340' side='right'caption='[[1p99]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P99 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
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{{STRUCTURE_1p99| PDB=1p99 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p99 OCA], [https://pdbe.org/1p99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p99 RCSB], [https://www.ebi.ac.uk/pdbsum/1p99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p99 ProSAT], [https://www.topsan.org/Proteins/MCSG/1p99 TOPSAN]</span></td></tr>
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</table>
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'''1.7A crystal structure of protein PG110 from Staphylococcus aureus'''
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H3JTG5_STAAM A0A0H3JTG5_STAAM]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Bacterial dipeptide ABC transporters function to import a wide range of dipeptide substrates. This ability to transport a wide variety of dipeptides is conferred by the cognate substrate binding protein (SBP) of these transporters. SBPs bind dipeptides with little regard for their amino acid content. Here, we report the 1.7 A resolution structure of lipoprotein-9 (SA0422) of Staphylococcus aureus in complex with the dipeptide glycylmethionine. Experimental characterization of the subcellular location of the protein confirmed that SA0422 is an acylated, peripheral membrane protein. This is the first structure determined for an SBP of a Gram-positive dipeptide ABC transporter. Usually, binding of dipeptides occurs in a binding pocket that is largely hydrated and able to accommodate the side chains of several different amino acid residues. Unlike any other known SBP, lipoprotein-9 binds the side chains of the glycylmethionine dipeptide through very specific interactions. Lipoprotein-9 shares significant structural and sequence homology with the MetQ family of methionine SBP. Sequence comparisons between MetQ-like proteins and lipoprotein-9 suggest that the residues forming the tight interactions with the methionine side chains of the ligand are highly conserved between lipoprotein-9 and MetQ homologues, while the residues involved in coordinating the glycine residue are not. Modeling of the Vibrio cholerae MetQ and lipoprotein-9 binding pockets can account for lipoprotein-9 substrate specificity toward glycylmethionine. For this reason, we have designated lipoprotein-9 GmpC, for glycylmethionine binding protein.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p99_consurf.spt"</scriptWhenChecked>
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1P99 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P99 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions., Williams WA, Zhang RG, Zhou M, Joachimiak G, Gornicki P, Missiakas D, Joachimiak A, Biochemistry. 2004 Dec 28;43(51):16193-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15610013 15610013]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p99 ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Staphylococcus aureus subsp. aureus mu50]]
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__TOC__
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[[Category: Joachimiak, A.]]
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</StructureSection>
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[[Category: Joachimiak, G.]]
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[[Category: Large Structures]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Staphylococcus aureus subsp. aureus Mu50]]
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[[Category: Schneewind, O.]]
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[[Category: Joachimiak A]]
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[[Category: Zhang, R.]]
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[[Category: Joachimiak G]]
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[[Category: Zhou, M.]]
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[[Category: Schneewind O]]
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[[Category: Mcsg]]
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[[Category: Zhang R]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Zhou M]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:50:34 2008''
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Current revision

1.7A crystal structure of protein PG110 from Staphylococcus aureus

PDB ID 1p99

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