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1pbg

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(New page: 200px<br /><applet load="1pbg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pbg, resolution 2.3&Aring;" /> '''THE THREE-DIMENSIONAL...)
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[[Image:1pbg.gif|left|200px]]<br /><applet load="1pbg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pbg, resolution 2.3&Aring;" />
 
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'''THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS'''<br />
 
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==Overview==
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==THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS==
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BACKGROUND: The enzyme 6-phospho-beta-galactosidase hydrolyzes, phospholactose, the product of a phosphor-enolpyruvate-dependent, phosphotransferase system. It belongs to glycosidase family 1 and no, structure has yet been published for a member of this family. RESULTS: The, crystal structure of 6-phospho-beta-galactosidase was determined at 2.3 A, resolution by multiple isomorphous replacement, using the wild-type enzyme, and a designed cysteine mutant. A second crystal form, found with the, mutant enzyme, was solved by molecular replacement, yielding the, conformation of two chain loops that are invisible in the first crystal, form. The active center, located through catalytic residues identified in, previous studies, cannot be accessed by the substrate if the two loops are, in their defined conformation. The enzyme contains a (beta alpha)8 barrel, and the relationship of its chain fold to that of other glycosidases has, been quantified. As a side issue, we observed that a cysteine point mutant, designed for X-ray analysis crystallized mainly as a symmetric dimer, around an intermolecular disulfide bridge formed by the newly introduced, cysteine. CONCLUSIONS: The presented analysis provides a basis on which to, model all other family 1 members and thereby will help in elucidating the, catalytic mechanisms of these sequence-related enzymes. Moreover, this, enzyme belongs to a superfamily of glycosidases sharing a (beta alpha)8, barrel with catalytic glutamates/aspartates at the ends of the fourth and, the seventh strands of the beta barrel.
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<StructureSection load='1pbg' size='340' side='right'caption='[[1pbg]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pbg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PBG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pbg OCA], [https://pdbe.org/1pbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pbg RCSB], [https://www.ebi.ac.uk/pdbsum/1pbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pbg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACG_LACLL LACG_LACLL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/1pbg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pbg ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6-phospho-beta-galactosidase 6-phospho-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.85 3.2.1.85] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PBG OCA].
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*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis., Wiesmann C, Beste G, Hengstenberg W, Schulz GE, Structure. 1995 Sep 15;3(9):961-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8535789 8535789]
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[[Category: 6-phospho-beta-galactosidase]]
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[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Schulz, G.E.]]
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[[Category: Schulz GE]]
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[[Category: Wiesmann, C.]]
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[[Category: Wiesmann C]]
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[[Category: SO4]]
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[[Category: hydrolase (glycosyl hydrolase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:39:53 2007''
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Current revision

THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS

PDB ID 1pbg

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