1pts

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(New page: 200px<br /><applet load="1pts" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pts, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE AND...)
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[[Image:1pts.gif|left|200px]]<br /><applet load="1pts" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pts, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN==
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The thermodynamic binding parameters and crystal structure for, streptavidin-peptide complexes where the peptide sequences were obtained, by random screening methods are reported. The affinities between, streptavidin and two heptapeptides were determined by titrating, calorimetric methods [Phe-Ser-His-Pro-Gln-Asn-Thr, Ka = 7944 (+/- 224), M-1, delta G degrees = -5.32 (+/- 0.01) kcal/mol, and delta H degrees =, -19.34 (+/- 0.48) kcal/mol; His-Asp-His-Pro-Gln-Asn-Leu, Ka = 3542 (+/-, 146) M-1, delta G degrees = -4.84 (+/- 0.03) kcal/mol, and delta H degrees, = -19.00 (+/- 0.64) kcal/mol]. The crystal structure of streptavidin, complexed with one of these peptides has been determined at 2.0-A, resolution. The peptide (Phe-Ser-His-Pro-Gln-Asn-Thr) binds in a turn, conformation with the histidine, proline, and glutamine side chains, oriented inward at the biotin-binding site. A water molecule is, immobilized between the histidine and glutamine side chains of the peptide, and an aspartic acid side chain of the protein. Although some of the, residues that participate in binding biotin also interact with the, screened peptide, the peptide adopts an alternate method of utilizing, binding determinants in the biotin-binding site of streptavidin.
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<StructureSection load='1pts' size='340' side='right'caption='[[1pts]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pts]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PTS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pts OCA], [https://pdbe.org/1pts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pts RCSB], [https://www.ebi.ac.uk/pdbsum/1pts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pts ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pts_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pts ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PTS OCA].
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*[[Avidin 3D structures|Avidin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin., Weber PC, Pantoliano MW, Thompson LD, Biochemistry. 1992 Oct 6;31(39):9350-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1390720 1390720]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
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[[Category: Group, Dupont.Protein.Crystallography.]]
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[[Category: Pantoliano MW]]
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[[Category: glycoprotein]]
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[[Category: Thompson LD]]
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[[Category: Weber PC]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:07:18 2007''
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Current revision

CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN

PDB ID 1pts

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